两种细胞增殖分析方法的比较性研究  

Contrastive study of two cell proliferation assays

作　　者：韩建群[1] 修瑞娟[1] 

机构地区：[1]中国医学科学院北京协和医学院微循环研究所卫生部微循环重点实验室,北京100005

出　　处：《山东医药》2012年第12期70-72,I0003,共4页Shandong Medical Journal

基　　金：北京协和医学院青年基金(imc-2010);公益性科研院所基本科研业务费专项基金(imc-2008)

摘　　要：目的比较MTT法和EdU标记法两种常用测定细胞增殖的方法的优劣。方法以化疗药物顺铂浓度梯度处理前列腺癌细胞株PC-3,分别采用MTT法和EdU标记技术进行细胞增殖变化测定。结果 MTT组顺铂浓度增至5μg/mL时细胞活力和增殖明显受抑(P<0.05);EdU掺入组顺铂浓度增至2μg/mL时增殖细胞阳性率和胞内荧光强度均显著下降(P<0.05)。结论 MTT法简单、快速、经济,结果与活细胞数有关,间接反映细胞的增殖状态;EdU标记技术灵敏、客观,特异性标记分裂期细胞,直接反映细胞的增殖。Objective To compare the detection sensitivity between MrlT and EdU incorporation for cell proliferation assay. Methods Prostate cancer cell line （PC-3） was exposed to different concentrations of cisplatin for 24 hours. Cells viability and proliferation were respectively detected by MTI＂ and EdU incorporation assay. Result PC-3 cell viability and proliferation were significantly decreased in 5 μg/mL condition by MTT detection （ P 〈 0.05 ）, however, the proliferation positive cell ratio and the density of fluorescence were significantly decreased in 2μg/mL condition by EdU incorporation assay （ P 〈 0.05 ）. Conclusion Simple, fast and economic are the advantages of MTI＂ method, but it only reflects the sta- tus of viable cells, independent with proliferation population; sensitivity, objectivity and specificity are the advantages of EdU incorporation assay, which is related with S phase cell cycle, directly reflects the cells proliferation.

关 键 词：细胞增殖 MTT EDU 

分 类 号：R331[医药卫生—人体生理学]