Complete mitogenome of the Lesser Purple Emperor Apatura ilia(Lepidoptera:Nymphalidae:Apaturinae) and comparison with other nymphalid butterflies  

柳紫闪蛱蝶线粒体基因组全序列及与相关蛱蝶类的比较分析(英文)

作　　者：陈梅[1] 田丽丽[1] 石庆会[1] 曹天文[2] 郝家胜[1] 

机构地区：[1]安徽师范大学生命科学学院分子进化与生物多样性研究室,安徽芜湖241000 [2]山西省农业科学院植物保护研究所昆虫研究室,山西太原030031

出　　处：《Zoological Research》2012年第2期191-201,共11页动物学研究（英文）

基　　金：supported by grants from the National Science Foundation of China (41172004,40871034);partially by the Provincial Key Projects of Natural Science Foundation,Colleges of Anhui Province (KJ2010A142)

摘　　要：The complete mitochondrial genome of Apatura ilia （GenBank accession no. JF437925） was determined as a circular DNA molecule of 15 242 bp, with common genes of 13 putative proteins, 2 rRNAs, and 22 tRNAs and of the same gene arrangement as in other sequenced lepidopterans. All protein-coding genes had the typical start codon ATN, except for the COI＇s using CGA as its start codon as previously demonstrated in other lepidopteran species. The comparison of the nucleotide sequences of the A. ilia mitogenome with ten other Nymphalidae species showed nearly identical gene orientation and arrangement, with only a few alterations in non-coding fragments. The nucleotide composition and codon frequency all fell into the range estimated for the order Lepidoptera. The A. ilia mitochondrial genome had the canonical set of 22 tRNA genes folded in the typical cloverleaf structure, with an unique exception of tRNAs^r （AGN）. The mitochondrial genes from A. ilia were overlapped in a total of 33 bp at 9 locations, as well as interleaved with a total of 155 bp intergenic spacers, spread over 12 regions with the size ranging from 1 to 49 bp. Furthermore, the spacer between ND6 and Cyt b harbored a microsatellite-like repeat （TA）23 not found in other completely sequenced nymphalid genomes. The 403 bp AT-rich region harbored two conserved motifs （ATAGA, ATTTA）, a 21 bp polyT stretch, a 10 bp poly-A region, along with two microsatellite-like repeats （ （TA）10 and （TA）7）, as detected in other nymphalid butterflies.该文对柳紫闪蛱蝶Apaturailia(鳞翅目:蛱蝶科)的线粒体基因组全序列进行了测定,同时结合其它已知蛱蝶类的相应序列进行了比较分析。结果显示:柳紫闪蛱蝶的线粒体基因组(GenBankaccessionno.:JF437925)是一个15242bp的环状DNA分子,包含13个蛋白质编码基因、2个rRNA基因和22个tRNA基因。13个蛋白编码基因中,除了COI基因的起始密码子是CGA外,其余12个蛋白编码基因都具有标准的ATN起始密码子;柳紫闪蛱蝶与其它已测的10种蛱蝶在基因定位和排列顺序方面几乎相同,只是在非编码序列上存在细微的差异,其核苷酸的构成及密码子使用频率都处于鳞翅目昆虫的范围之内。22个的tRNA基因中,除了tRNASer(AGN)缺少DHU臂,其余的tRNA基因都显示为典型的三叶草结构。基因组共存在9处基因间重叠区(总长度为33bp)以及12个基因间隔区(总长为155bp,最长间隔是49bp,最短的是1bp)。在ND6和Cytb间的间隔区中还发现有(TA)23似微卫星结构。与其他蛱蝶类相似,403bp的AT富集区包含有ATAGA,ATTTA二个保守模块(一个21bp的poly-T,一个10bp的poly-A),以及二个似微卫星的重复结构((TA)10和(TA)7)。

关 键 词：Mitochondrial genome LEPIDOPTERA NYMPHALIDAE Apatura ilia 

分 类 号：Q963[生物学—昆虫学]