基于唾液的拉曼光谱检测进行肝癌诊断的研究  

Study on Diagnosis of Liver Cancer by Surface Enhanced Raman Spectroscopy on Human Saliva

作　　者：崔子健[1] 姚雨露[1] 王艳[1] 陈安宇[1] 刘春伟 郭浔 汪泓 

机构地区：[1]首都医科大学生物医学工程学院,北京100069 [2]美国欧普图斯公司

出　　处：《光散射学报》2012年第1期40-47,共8页The Journal of Light Scattering

基　　金：北京市教育委员会科技计划重点项目(KZ201010025018)

摘　　要：表面增强拉曼光谱技术是近年来快速发展的一种痕量特征标记性物质检测技术,达到了分子识别水平,具有很高的灵敏度,能够精确地分析和了解物质结构和成分。人体唾液中含有大量体征相关的特异性蛋白质和分泌物质。唾液检测具有无创、获取方便、适合开展大规模普查等优点。表面增强拉曼光谱检测技术可以快速地对唾液进行检测,二者的结合,有可能成为一种癌症诊断的新方法。本文利用表面增强光谱技术对肝癌患者进行实验研究,通过对肝癌患者唾液的采集、样品的处理、光谱的采集及光谱分析,肝癌特异性标记物AFP(alpha fetoprotein)的检测及分析等过程得到肝癌相关特征信息,表明肝癌患者与正常人的唾液样本分类良好,灵敏度及特异性较好;进一步验证了AFP抗原的分子组成,且AFP抗原的拉曼强度基本随浓度的递增呈递减趋势;对肝癌患者的唾液样本及AFP抗原的拉曼光谱进行比对分析,提示利用表面增强拉曼光谱检测唾液有可能诊断早期癌症。Surface Enhanced Raman Spectroscopy （SERS） is a trace amount substance de- tecting technique developing quickly in recent years. It is a physical method at molecular levels, and it has high sensitivity in analyzing structure, component and content of sub- stances. Human saliva contains abundant proteins and substances. Many diseases can be diagnosed by analyzing saliva. Saliva can be collected conveniently and saliva examination is non-invasive which is appropriate for large-scale health surveys. This paper using the SERS technology to the liver cancer patients, though collecting and processing the saliva sample of liver cancer patients, acquires the spectra of the saliva sample and analyzing the spectra of the specific marker AFP of liver cancer. The Results stated that the spectra of saliva samples of liver patients can be separated from healthy controls and the result showhigh sensitivity and specificity. Further certificated the molecular forms of AFP, and the Raman intensity of AFP declines with its increasing concentrations. The spectra of saliva samples, serum samples and the AFP antigen were comparatively analyzed the results demonstrated that it would be an effective method for early diagnosis of liver cancer by SERS on saliva.

关 键 词：拉曼光谱 唾液 AFP 肝癌诊断 

分 类 号：O433.4[机械工程—光学工程] R331[理学—光学]