毛白杨12种microRNAs的低温胁迫差异表达分析  

Differential Expression Analysis of 12 MicroRNAs Under Cold Stress in Populus tomentosa

作　　者：张译云[1] 任媛媛[1] 陈磊[1] 徐吉臣[1] 张志毅[1] 王延伟[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室/国家林业局树木花卉育种与生物工程重点开放实验室,北京100083

出　　处：《中国农学通报》2012年第7期1-7,共7页Chinese Agricultural Science Bulletin

基　　金：国家林业公益性行业科研专项项目"重要乡土树种核心种质评价及高效育种共性技术研究"(201004009);北京林业大学新进教师科研启动基金项目"杨树抗低温相关miRNA克隆及功能分析"(BLX2009005)

摘　　要：为了探讨microRNAs(miRNAs)在杨树低温胁迫不同时间段的差异表达规律,初步鉴定参与杨树低温胁迫响应的miRNAs,以重要乡土树种毛白杨为材料,进行不同时间段(0、8、14、20h)的低温胁迫(4℃)处理,分别提取小片段RNAs(<200nt),利用实时定量PCR技术研究毛白杨在低温胁迫后12种miRNAs的差异表达规律。结果显示,miRNAs的表达在低温胁迫下有显著变化,且呈现动态反应。在低温胁迫下,大部分miRNAs的表达受到抑制。miR168a、miR169ac、miR394a-3p、miR530a的表达量在各个时间段显著下调。该研究初步表明,miRNAs在调控毛白杨对低温胁迫的反应中起着重要作用。In order to study the differential expression of microRNAs （miRNAs） in Populus treated with cold stress for different time and identify cold stress related miRNAs, in this study, Populus tomentosa plantlets were treated for different time （0, 2, 8, 14, 20 h） under cold stress （4℃）. Small-sized RNAs （〈200 nt） were extracted as template, real-time PCR was used to detect the differential expression of 12 miRNAs under cold stress. The results showed that significant changes of miRNAs were found under different cold stress and the dynamic expression was detected. The expression of most 12 miRNAs was suppressed under cold stress. The expression of miR168a, miR169ac, miR394a-3p, miR530a was down-regulated significantly at all the stress time. The study showed that miRNAs played critical roles in cold stress.

关 键 词：MICRORNAS 实时定量PCR 毛白杨 表达分析 

分 类 号：S722[农业科学—林木遗传育种]