异土木香内酯对金黄色葡萄球菌肠毒素表达的影响  

Isoalantolactone Reduces Enterotoxins Production by Staphylococcus Aureus

作　　者：吴金梅[1,2] 邱家章[1] 邓旭明[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]郑州牧业工程高等专科学校,郑州450002

出　　处：《中国农学通报》2012年第8期51-55,共5页Chinese Agricultural Science Bulletin

基　　金：重庆市科技创新能力建设项目"甘草查尔酮A对金葡菌的作用研究"(CSTC-2009CB1010)

摘　　要：为了考察异土木香内酯对金黄色葡萄球菌的抗菌活性,并研究其对金葡菌肠毒素表达的影响。应用肉汤微量稀释法测定异土木香内酯对金黄色葡萄黄色球菌标准株的最低抑菌浓度,采用Western-blot分析、蛋白酶试验、肿瘤坏死因子释放试验和荧光定量PCR分析测定异土木香内酯对金黄色葡萄黄色球菌主要肠毒素SEA和SEB表达的影响。结果表明:异土木香内酯在2048μg/mL仍然不能抑制金葡菌的生长,然而低浓度(1～8μg/mL)的异土木香内酯能够剂量依赖性地降低金葡菌SEA和SEB的表达。In the study,we were aimed to investigate the antimicrobial activity of isoalantolactone against Staphylococcus aureus,and further assess the influence of isoalantolactone on the expression of enterotoxins by S.aureus.A broth micro-dilution method was used to determine the minimal inhibitory concentrations（MICs）;Western-blot,proteolytic activity,TNF-α release and real-time RT-PCR assays were performed to assess the effects of isoalantolactone on S.aureus SEA and SEB expression.The data showed that the MICs of isoalantolactone against S.aureus strains were greater than 2048 μg/mL.However,low concentrations of isoalantolactone（1-8 μg/mL） could dose-dependently inhibit the expression of SEA and SEB by S.aureus.

关 键 词：金黄色葡萄球菌 肠毒素 异土木香内酯 

分 类 号：S859.7[农业科学—临床兽医学]