低温高湿对转Bt基因抗虫棉杀虫蛋白表达及其氮代谢的影响  

Effect of Low Temperature and High Humidity on Leaf Bt Protein Content and Nitrogen Metabolism of Bt Cotton

作　　者：张明伟[1] 缪军[1] 顾超[1] 吕春花[1] 陈德华[1] 张祥[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《中国农学通报》2012年第9期218-221,共4页Chinese Agricultural Science Bulletin

基　　金：扬州大学大学生学术科技创新基金;扬州大学科技创新培育基金(2010CXJ036);转基因生物新品种培育科技重大专项(2009ZX08013-011B);转基因棉花新品种产业化(2009ZX08013-014B);江苏省"青蓝工程"学术带头人培养基金;国家自然科学基金(30971727;31171479)

摘　　要：通过对2个不同类型Bt棉品种不同生育期低温高湿胁迫研究表明,低温高湿胁迫48 h整个生育期叶片Bt杀虫蛋白含量都下降,其中在结铃期,Bt杀虫蛋白含量大幅下降。低温高湿使叶片GPT活性下降,游离氨基酸含量、可溶性蛋白含量下降,即氮代谢减弱。The experiment of both low temperature and high humidity on the two different types of Bt cotton cultivars was conducted at different developing periods,The results showed that,the low temperature at both 18℃ and high humidity（90%-95% RH） for 48 hours resulted in leaf Bt protein content decreased in three periods,especially in the peak boll period.The leaf GPT activity,the contents of leaf amino acid and soluble protein decreased during peak boll period under the both low temperature and high humidity treatment.It had characteristics of weak N metabolism intensity.

关 键 词：BT棉 低温 高湿 BT蛋白 氮代谢 

分 类 号：S317[农业科学—作物栽培与耕作技术]