自拟通肺平哮汤对哮喘豚鼠Th1/Th2细胞功能调节作用的影响  

Effect of Tongfeipingxiao decoction on regulating function of Th1/Th2 cell in guinea pig with asthma

作　　者：罗光伟[1] 徐玮[2] 吴霜[3] 黄燕玲[1] 杨硕[1] 毛莉娜[1] 黄小龙[1] 张念[1] 徐清[1] 孙洁民[1] 肖宏[4] 

机构地区：[1]武汉市中西医结合医院呼吸内科,430022 [2]湖北中医药大学 [3]云南中医学院 [4]武汉市中西医结合医院中心实验室,430022

出　　处：《疑难病杂志》2012年第4期282-284,F0003,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：武汉市卫生局资助课题(No.武卫[2001]235号)

摘　　要：目的探讨中药复方通肺平哮汤对哮喘豚鼠气道炎性反应的抑制及对Th1/Th2细胞功能调节作用的影响。方法 40只健康雄性Hartley系豚鼠随机分为空白对照组、病理模型组、地塞米松组、通肺平哮汤组各10只,后3组用卵白蛋白(OVA)致敏,建立哮喘豚鼠模型,然后分别给予蒸馏水(2 ml/只)、地塞米松(0.01 g/kg)、通肺平哮汤(20 g/kg),用药7 d后处死动物,采用流式细胞术分析CD_4^+细胞干扰素-γ(IFN-γ)和白介素-4(IL-4)水平变化。结果与空白对照组比较,病理模型组IFN-γ水平明显降低,IL-4水平明显升高,差异有统计学意义(P<0.05);与病理模型组比较,地塞米松组、通肺平哮汤组IFN-γ水平明显升高(P<0.05),而IL-4水平无明显差异(P>0.05);地塞米松组与通肺平哮汤组比较差异亦无统计学意义(P>0.05)。病理组织学检查示病理模型组气道壁及肺间质大量嗜酸性粒细胞浸润,平滑肌层增厚,管腔狭窄,地塞米松组及通肺平哮汤组病理改变显著减轻。结论通肺平哮汤可以抑制Th2的活化,使Th1/Th2平衡向Th1偏移从而发挥平喘作用。Objective To investigate the effect of Tongfeipingxiao decoction on regulating function of Thl/Th2 cell in guinea pig with asthma.Methods 40 healthy male Hartley guinea pigs were randomly allocated into four groups as follows： control group,model group,dexamethasone treatment group and Tongfeipingxiao decoction treatment group.The asthma model was induced by intra-peritoneal injection of ovalbumin.Then distilled water（2 ml per animal）,dexamethasone（0.01g/ kg）,Tongfeipingxiao（20g/kg） were given in each group respectively.The animals were sacrificed after 7 days of treatment. The level of CD_4~＋ IFN-7 and IL-4 were tested by flow cytometer.Results Compared with control group,IFN-γwere decreased and IL-4 increased obviously in the Tongfeipingxiao decoction group（P 0.05）.Compared with model group,IFN-γwas increased（P 0.05） and IL-4 did not reveal obviously differences in dexamethasone and Tongfeipingxiao groups （P 0.05 ）.Pathological results revealed that the disease were released obviously in the dexamethasone and Tongfeipingxiao groups.Conclusion Tongfeipingxiao decoction could release the symptom of asthma according to inhibiting the activation of Th2 and let Th1/Th2 balance shift to Th1.

关 键 词：哮喘 通肺平哮汤 TH1/TH2 流式细胞术 豚鼠 

分 类 号：R285.5[医药卫生—中药学]