1个控制水稻绿苗分化率QTL的效应验证  

Effect of a plant regeneration frequency QTL in rice

作　　者：李菁[1] 林拥军[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2012年第3期270-274,共5页Journal of Huazhong Agricultural University

基　　金：抗虫转基因水稻新品种培育专项(2008ZX08001-001)

摘　　要：HJ-37是日本晴(供体)/珍汕97(受体)通过连续回交所构建的重叠导入系中的一个家系,该家系在其导入片段的区域内部存在一个与绿苗分化率相关的QTL。以HJ-37与珍汕97B杂交后的F2代群体为研究对象,采用SSR分子标记筛选重组单株与绿苗分化率检测相结合的办法来对此QTL进行效应验证并缩短该QTL的距离。最终,10号株系绿苗分化率平均值为53.13%,t值为4.897 6,此数据表明该株系的绿苗分化率与对照相比明显升高。同时该QTL在遗传图谱上的距离缩小到了RM3289-RM6295区段内。该研究为最终分离克隆提高绿苗分化率的相关基因打下了基础。HJ-37 was developed through backcross and marker-aided selection by using japonica variety Nipponbare as donor parent and indica variety Zhenshan 97B as recurrent parent.F2 seeds from crossing HJ-37 with Zhenshan 97B were used to select the recombinant plants with SSR markers.Then,the homozygous seeds from the recombinant plants were used to test the plant regeneration frequency.Meanwhile,SSR was utilized to map the recombinant sites.Finally,the effect of this QTL was verified.Average of plant regeneration frequency and t value of plant 10 is 53.31% and 4.897 6.This data shows plant 10 is obviously improved in PRF.Meanwhile,the distance of QTL was narrowed to the segment between SSR markers RM3289 and RM6295.The result will be useful for isolating and cloning the gene of high frequency of plant regeneration.

关 键 词：水稻 重叠导入系 SSR 重组单株 绿苗分化率 

分 类 号：S511.503[农业科学—作物学]