西瓜细菌性果斑病菌在不同场所存活期的检测  

Detection on survival time in different places of Acidovorax avenae subsp.citrulli

作　　者：李艳嫦[1] 孔静月[1] 吴九玲 王琳[3] 任小平[3] 刘琼光[1] 

机构地区：[1]华南农业大学资源环境学院,广州510642 [2]广东省海丰县农业局,海丰516400 [3]广东省农业厅植检站,广州510500

出　　处：《华中农业大学学报》2012年第3期332-336,共5页Journal of Huazhong Agricultural University

基　　金：农业部公益性行业专项(201003066-1);广东省植物防疫检疫研究专项(4200-F09043)

摘　　要：选择西瓜细菌性果斑病菌(Acidovorax avenae subsp.citrulli,Aac)的抗利福平菌株RifAac,通过人工模拟接种试验,采用选择性平板分离、菌落PCR(Bio-PCR)和常规PCR方法,观察该病原细菌在西瓜种子、土壤、病残体和田水中的存活期。结果表明,果斑病细菌在种子表面可存活4～5个月,在田水中可存活2个月,在不含植物残体的土壤中可存活8个月,在含西瓜残体的土壤中可以存活12个月以上。Bacterial fruit blotch（BFB） of cucurbits,caused by Acidovorax avenae subsp.citrulli（Aac）,is one of the most devastating diseases of watermelon and muskmelon.Fewer studies were done on its survival time in different places.In this study,the survival time of Aac inoculated with the anti-fampicin strain RifAac in seed,soil debit and water was investigated by isolation,bio-PCR and PCR.The results showed that Aac could survive in the watermelon seeds for 4-5 months,in the water for 2 months,in the soil for 8 months and within the soil with debris for longer than one year.

关 键 词：西瓜细菌性果斑病 燕麦嗜酸菌西瓜亚种 存活期 菌落PCR 检测 

分 类 号：S432.42[农业科学—植物病理学]