3种养殖模式水体中细菌多样性研究  

Biodiversity of aquatic microbial communities in three aquacultural systems

作　　者：李革雷[1] 陈昌福[1] 高宇[1] 刘振兴[1] 谭晶晶[1] 

机构地区：[1]华中农业大学水产学院,武汉430070

出　　处：《华中农业大学学报》2012年第3期381-390,共10页Journal of Huazhong Agricultural University

基　　金：农业公益性行业科研专项经费项目(200803013);973项目(2009CB118700)

摘　　要：采用16SrDNA克隆文库技术,对位于武汉市少潭河水库养殖基地3种养殖模式[根据放养鱼所占比例,草鱼75%、鲢15%、鳙6%、异育银鲫4%,为模式1(MSH1);草鱼75%、鲢15%、鳙3%、匙吻鲟3%、异育银鲫4%,为模式2(MSH2);草鱼75%、鲢15%、匙吻鲟6%、异育银鲫4%,为模式3(MSH3)]水体中细菌多样性进行研究。系统发育分析结果表明:3种养殖模式中细菌的16SrDNA克隆文库的序列总共分布于10个门,分别是变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、蓝藻细菌门(Cyanobacteria)、浮霉菌门(Planctomycetes)、疣微菌门(Verrucomicrobia)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)、梭杆菌门(Fusobacteria)和OP10。对3种养殖模式中细菌的16SrDNA克隆文库的序列多样性分析结果表明,MSH2中细菌克隆文库的多样性指数优于MSH1和MSH3,而MSH3优于MSH1。The bacterial diversity in three aquacultural systems in Shaotan river reservoir of Wuhan city was studied by the method of 16S rDNA clone library.The grass crap,silver carp,and gible carp were basal groups of the 3 aquacultural systems,while the paddlefish and big head carp were added by different proportions.No paddlefish and 6% big head carp was named system 1,while 3% paddlefish and 3% big head carp was named system 2,and 6% paddlefish and no big head carp was named system 3.The result of phylogenetic analysis showed that the sequences of 16S rDNA clone library in three aquacultural systems included 10 bacterial communities：Proteobacteria,Bacteroidetes,Cyanobacteria,Planctomycetes,Verrucomicrobia,Actinobacteria,Acidobacteria,Chloroflexi,Fusobacteria and OP10.The multifarious analysis demonstrated that the diversity index of system 2 was better than the other aquacultural systems,while the diversty index of system 3 was better than system 1.

关 键 词：养殖模式 16SrDNA克隆文库 细菌多样性 系统发育分析 

分 类 号：S917[农业科学—水产科学]