高效毛细管电泳法同时分离氟喹诺酮类与四环素类药物的研究  

Separation and determination of fluoroquinolones and tetracyclines by high performance capillary electrophoresis

作　　者：沈虎琴[1] 檀华蓉[2] 祁克宗[3] 田春秋[1] 

机构地区：[1]安徽农业大学茶与食品科技学院,合肥230036 [2]安徽农业大学生物技术中心,合肥230036 [3]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2012年第2期207-210,共4页Journal of Anhui Agricultural University

基　　金："十一五"国家科技支撑项目(2006BAK02A08_4)资助

摘　　要：采用高效毛细管电泳建立4种氟喹诺酮类（FQS）与2种四环素类（TC）药物同时分离的方法。通过考虑缓冲液离子浓度、pH、有机添加剂以及分离电压、温度等电泳参数,通过正交试验确立了电泳的最佳条件：紫外检测波长270 nm,缓冲溶液为50 mmol·L^-1硼砂-200 mmol·L^-1硼酸,pH 8.41,分离电压为18 kV。结果表明,6种药物在12 min内达到完全分离,且各组分浓度与峰面积呈良好的线性关系,其相关系数R^2为0.998 2-1,迁移时间的相对标准偏差为0.25%-0.68%,峰面积相对标准偏差为7.3%-9.8%。A method for the simultaneous separation of fluoroquinolones（FQs）and tetracyclines（TCs）by high performance capillary electrophoresis（HPCE） was developed.The effects of several factors such as the con-centration and pH of running buffer,the organic added to the electrolyte,the voltage and the temperature on sepa-ration were investigated.The optimal conditions were as follows.The UV detection wavelength was 262 nm.The running butter consisted of 50 mmol·L^-1 NaB4O7-200 mmol·L^-1 H3BO3 with pH 8.41,and the separation voltage was at 18 kV.The results showed that the six remedies can be well separated within 12 min.Each had a good lin-ear relationship（R^2=0.9982-1）.The RSD was 0.25%-0.68% and 7.3%-9.8%.

关 键 词：高效毛细管电泳 氟喹诺酮 四环素 同时分离 

分 类 号：S859.83[农业科学—临床兽医学]