簇毛麦Dvblt101基因及其启动子的克隆与序列分析  

Cloning and sequence analysis of the Dvblt101 gene and it's promoter from Dasypyrum villosum

作　　者：何华纲[1] 潘薇[1] 任新龙[1] 孙卫红[1] 朱姗颖[2] 别同德[3] 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013 [2]江苏大学环境学院,镇江212013 [3]扬州市农业科学研究院,扬州225007

出　　处：《安徽农业大学学报》2012年第2期269-272,共4页Journal of Anhui Agricultural University

基　　金：江苏省普通高校研究生科研创新计划(CX09B_211Z);江苏省自然科学基金项目(BK2009175;BK2010344);现代农业产业技术体系项目(NYCYTX-03);江苏高校优势学科建设工程资助项目共同资助

摘　　要：BLT101家族是一类高度保守的小分子质膜蛋白,在植物抗寒、耐旱、耐盐、脱落酸(ABA)应答等过程中起重要作用。利用PCR技术从抗寒、耐旱的簇毛麦中克隆了Dvblt101基因及其启动子。结果表明,Dvblt101cDNA全长422 bp,具有完整的开放阅读框(ORF),编码一条54个氨基酸残基的多肽,并具有2个保守的跨膜结构域;Dvblt101基因长为754 bp,含有2个外显子和1个内含子,启动子区域具有2个干旱/冷应答核心元件(DRE/CRT)、2个MYC结合位点和3个ABA应答元件(ABRE),该基因对低温、干旱等逆境及ABA可能存在广泛的应答。本研究为进一步揭示BLT101家族在簇毛麦抗逆过程中的作用机理奠定了基础。BLT101 family,a kind of conserved low molecular protein sublocalized in plasma membrane,play important roles in cold acclimation,drought tolerance and abscisic acid（ABA） response in plant.By PCR method,a Dvblt101 gene and its＇ promoter were cloned from Dasypyrum villosum,a closely relative to common wheat.Dvblt101 cDNA is 422 bp in length with an open reading frame（ORF） encoding 54 amino acid residues,which contains two conserved transmembrane domains.The genomic DNA of Dvblt101 gene is 754 bp in length with two extrons and one intron.In the promoter region,two DRE/CRT,two MYC-binding sites and three ABA re-sponse elements（ABRE） are predicted.Dvblt101 gene of D.villosum might be widely response to low tempera-ture,drought,ABA and other abiotic stresses.These results could contribute to the understanding of abiotic stress tolerance mechanism in D.villosum.

关 键 词：簇毛麦 Dvblt101基因 启动子 序列分析 

分 类 号：Q751[生物学—分子生物学]