杏仁中央核μ阿片受体参与蔗糖溶液摄入的调节  

μ-opioid receptors in the central nucleus of the amygdala modulate sucrose solution intake in rats

作　　者：孙波[1] 闫剑群[1] 王倩[1] 赵小林[1] 李金容[1] 闫薇[1] 陈坷[1] 杨雪娟[1] 赵师儒[1] 闫君宝[1] 

机构地区：[1]西安交通大学医学院生理及病理生理系//教育部环境与疾病相关基因重点实验室,陕西西安710061

出　　处：《南方医科大学学报》2012年第4期487-491,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30970973;31000518;31171052);陕西省自然科学基金青年项目(2011JQ4010)~~

摘　　要：目的探讨杏仁中央核内μ阿片受体是否参与大鼠蔗糖溶液摄入的调节及其可能的机制。方法杏仁中央核内注入μ阿片受体激动剂DAMGO或生理盐水,测量大鼠在双瓶选择试验中对蔗糖溶液及蒸馏水的摄入量;利用荧光免疫组织化学方法,在大鼠摄入蔗糖溶液或蒸馏水后,观察杏仁中央核内μ阿片受体/Fos免疫阳性双标记神经元的数量。结果与生理盐水注射组相比,杏仁中央核内注入DAMGO增加了大鼠3 h内的蔗糖溶液摄入量;与蒸馏水摄入组相比,大鼠摄入蔗糖溶液后,杏仁中央核内c-Fos阳性神经元及μ阿片受体/Fos共表达神经元均显著增加(P<0.05)。结论杏仁中央核参与大鼠蔗糖溶液摄入的调节,该调节作用可能部分是由μ阿片受体介导的。Objective To explore the role of μ-opioid receptors（MOR） in the central nucleus of the amygdala（CeA） in modulating sucrose solution intake in rats.Methods Sprague-Dawley rats received intra-CeA injection of MOR agonist DAMGO or saline,and then underwent two bottle choice test between sucrose solution and distilled water.After intake of sucrose solution or distilled water,activated neurons in the CeA were labeled and identified with MOR/Fos-double labeling immunohistochemistry.Results Compared with saline injection,intra-CeA injection of DAMGO significantly increased sucrose solution intake in rats over a 3-h period.Sucrose solution intake induced significantly more c-Fos and MOR/Fos double-labeled neurons in the CeA than distilled water intake.Conclusion The CeA participates in modulation of sucrose intake in rats,and MOR may partly mediate this mechanism.

关 键 词：杏仁中央核 Μ阿片受体 FOS 蔗糖溶液摄入 

分 类 号：R339.13[医药卫生—人体生理学]