芥菜籽对小鼠变应性接触性皮炎的抑制作用  

Effects of mustard seed on 2,4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice

作　　者：汶春苗[1] 杨闰平[1] 胡坚[1] 焦泽龙 杨宇光[1] 杨剑凌 李恒进[1] 赵华[1] 

机构地区：[1]解放军总医院皮肤科,北京100853 [2]海军机关直属门诊部皮肤科,北京100039 [3]解放军第513医院皮肤科,甘肃兰州732750

出　　处：《南方医科大学学报》2012年第4期569-572,共4页Journal of Southern Medical University

基　　金：解放军总医院苗圃基金(01020604)

摘　　要：目的研究芥菜籽对小鼠变应性接触性皮炎(ACD)的抑制作用,探讨其可能的作用机制。方法将18只BALB/c小鼠随机分为模型组、芥菜籽组、正常组,模型组和正常组喂养普通饲料,芥菜籽组喂养含5%芥菜籽饲料。3周后,以2.4-二硝基氟苯(DNFB)诱导小鼠ACD模型,耳部激发24 h后,观察芥菜籽对小鼠耳肿胀度,组织病理改变,皮损处朗格汉斯细胞(LC)浸润、局部促炎细胞因子及血清IFN-γ等因子的水平。结果芥菜籽能抑制ACD小鼠的耳肿胀度、降低皮损处LC浸润、降低IL-1β、TNF-α、IL-6等炎症因子的表达(P<0.05),但对血清IFN-γ、IL-4、IL-17水平没有影响(P>0.05)。结论芥菜籽对小鼠ACD有抑制作用,可能与其抑制LC的迁移及降低炎症因子有关。Objective To investigate the therapeutic effect of mustard seed on allergic contact dermatitis（ACD） in mice and explore the mechanism.Methods Eighteen BALB/c mice were randomly divided into normal control group,model group and mustard seed group.The mice in the normal control group and model group were fed with normal chow,and those in mustard seed group were given 5% mustard seed mixed in the chow.Three weeks later,ACD was induced on the ear using 2,4-dinitrofluorobenzene.After 24 h,the swelling of the ear was examined,and the rats were sacrificed to collect the ear tissue ears and blood for histopathological and immunohistochemical examinations,RT-PCR and enzyme-linked immunosorbent assay.Results In mice with ACD,feeding with mustard seeds significantly lessened the ear swelling,improved the tissue histopathology,lowered the number of infiltrating Langerhans cells,and reduced the expressions of IL-1β,TNF-α and IL-6 mRNA in the ear,but did not cause significant changes in serum levels of IL-4,IFN-γ and IL-17.Conclusion Mustard seed inhibits ACD in mice possibly by suppressing the expressions of IL-1β,TNF-α and IL-6 mRNA and inhibiting Langerhans cell migration in the epidermis.

关 键 词：芥菜籽 二硝基氟苯 变应性接触性皮炎 朗格汉斯细胞 

分 类 号：R285.5[医药卫生—中药学]