木薯叶片染色体制片技术研究  

Technique for Microscopical Slides of Cassava Leaf for Chromosome Observation

作　　者：张健[1,2] 郭军辉[1,2] 陈雄庭[2] 王颖[2] 王文泉[2] 赖杭桂[1] 吴坤鑫[2] 倪彦艳[1,2] 黄琴[1,2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2012年第1期20-23,共4页Chinese Journal of Tropical Crops

基　　金：国家"973"计划"木薯淀粉高效累积途径及其关键基因"项目(No.2010CB126601)

摘　　要：以木薯嫩叶为材料,对其染色体制片技术的取样时间、预处理药剂、固定液、解离方法以及染色剂等方面进行了试验研究。结果表明：取材时间为上午8：30~10：00,用0.1%秋水仙素与0.002 mol/L 8-羟基喹啉混合液室温预处理3 h,经固定液（无水酒精∶氯仿∶冰醋酸=6∶3∶1）固定,用1 mol/L盐酸60℃下解离8 min,再用改良苯酚品红染色压片镜检。The young leaves of Manihot esculenta Crantz were used to study the effects of sampling time,pretreatment chemical solution,fixed solutions,hydrolysing time and staining on the chromosome observation slides.The results showed that the optimal sampling time of leaves was about 8：30~10：00 a.m.The samples pretreated with a solution of 0.1% colchicine and 0.002 mol/L 8-hydroxyquinoline at room temperature for 3 hours and fixed with the fixative solution（alcohol ∶ chloroform ∶ glacial acetic acid = 6 ∶ 3 ∶ 1）,and then hydrolysed with 1 mol/L HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution were proved to be nice for the microscopical slides preparation of Cassava Leaf for Chromosome Observation.

关 键 词：木薯 嫩叶 染色体 制片技术 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]