魔芋高效再生体系的建立与优化  

Establishment of an Efficient Regeneration System of Amorphophallus konijac

作　　者：孙继华[1] 杨超[2,3] 刘菊华[2] 张静[2] 

机构地区：[1]中国热带农业科学院科技信息研究所,海南海口571101 [2]农业部热带作物生物技术重点开放实验室中国热带农业科学院热带生物技术研究所,海南海口571101 [3]海南省嘉积中学,海南琼海571400

出　　处：《热带作物学报》2012年第1期84-88,共5页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务费专项(No.ITBB110202)

摘　　要：研究不同激素及浓度配比对魔芋愈伤组织、不定芽和根诱导的影响。结果表明,诱导愈伤组织的最佳激素组合为MS+6-BA 0.5 mg/L+NAA 0.5 mg/L,其诱导率为82%;诱导不定芽分化的最佳配方为MS+6-BA 1.5 mg/L+NAA 0.5 mg/L,其分化率为490%;诱导生根的最佳激素组合配方为MS+NAA 0.5 mg/L+6-BA 1.0 mg/L,其生根率为90%。Aimed at establishing an efficient regeneration system for Amorphophallus konijac and providing a theoretical foundation for large-scale production in factories,the effects of different phytohormones on callus,bud and root were investigated.The results indicated that the optimum culture medium of callus initiation was MS＋6-BA 0.5 mg/L＋NAA 0.5 mg/L and the regeneration rate was 82%.The optimum culture medium of bud differentiation was MS＋6-BA 1.5 mg/L＋NAA 0.5 mg/L and the regeneration rate was 490%.The optimum culture medium of rooting was MS＋NAA 0.5 mg/L＋6-BA 1.0 mg/L and the rooting frequency was 90%.

关 键 词：魔芋 组织培养 高效再生 

分 类 号：S283[农业科学—农业水土工程]