内侧内嗅皮层微注射Orexin-A对大鼠睡眠-觉醒及空间记忆的影响  

Effect of Orexin-A injected into medial entorhinal cortex of rats on sleep-wakefulness and spatial memory

作　　者：齐爱萍[1] 何超[1] 夏建霞[1] 张丹[1] 陈芳[1] 阎洁[1] 胡志安[1] 

机构地区：[1]第三军医大学基础医学部生理学教研室,重庆市神经科学研究所,重庆400038

出　　处：《第三军医大学学报》2012年第8期715-718,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(81071074)~~

摘　　要：目的探讨内嗅皮层注射Orexin-A对Sprague-dawley(SD)大鼠睡眠-觉醒周期以及对空间记忆的影响。方法选用健康成年雄性SD大鼠45只,体质量250～300 g,采用同步记录大鼠脑电和肌电活动,观察Orexin-A及Orexin1受体阻断剂SB-334867内侧内嗅皮层微注射后大鼠睡眠-觉醒周期变化。采用T-迷宫交替选择次数,观察SB-334867内侧内嗅皮层微注射后大鼠T-迷宫选择正确率的变化。结果以人工脑脊液(ACSF)、二甲亚砜(DMSO)自身对照相比,分别给予大鼠内侧内嗅皮层双侧微注射Orexin A及SB-334867 0.5μl后3 h(11:00-12:00、12:00-13:00、13:00-14:00)觉醒周期时间无影响(P>0.05),给药后24 h觉醒周期时间也无显著差异(P>0.05)。微量注射药物后,Orexin-A(0.1 nmol/0.5μl)及SB-733467(6 mmol/0.5μl)在11:00-14:00 3 h内对觉醒时间、NREM时间及REM时间均无影响(P>0.05),即对3 h内(11:00-14:00)大鼠总睡眠觉醒时间无显著性差异(P>0.05)。与DMSO组比较大鼠内侧内嗅皮层双侧微注射SB-334867 0.5μl后,T-迷宫交替选择正确次数明显减少,正确率下降,有显著性差异(P<0.001)。结论内侧内嗅皮层Orexin系统的支配可能参与了空间记忆,而不参与睡眠-觉醒周期调节。Objective To study the effect of Orexin-A injected into medial entorhinal cortex （MEC） of rats on sleep-wake cycle and spatial memory of SD rats. Methods Forty-five healthy adult male SD rats, weighing 250 to 300 g, were included in this study. Their cerebral and myoelectrical activities were synchronically recorded, their sleep-wake cycle and accuracy rate of alternative selection in T-maze were observed after injection of Orexin-A and SB-334867. Results Oexin-A and SB-334867 showed no significant effect on sleepwake cycle, NREM and REM sleep time of rats 3 h after they were injected into MEC of rats compared with that of ACSF and DMSO （P 〉 0.05 ）. However, the accuracy rate of alternative selection in T-maze was significantly lower after injection of SB-733467 than after injection of DMSO（P 〈0. 001 ）. Conclusion The Orexin system in MEC may be involved in spatial memory but not in sleep-wake cycle.

关 键 词：Orexin—A 内侧内嗅皮层 空间记忆 

分 类 号：R341[医药卫生—基础医学]