血清淀粉样P蛋白缺失对小鼠动脉粥样硬化斑块形成的影响  

Effect of serum amyloid P component deficiency on formation of atherosclerosis plaques in mice

作　　者：李小强[1] 王尽淘[1] 桂亚丽[1] 唐富天[1] 兰天[1] 温定文[1] 吴腾[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广州510006

出　　处：《第三军医大学学报》2012年第8期734-737,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(973计划;2010CB529702);国家自然科学基金(30900764)~~

摘　　要：目的观察血清淀粉样P蛋白(serum amyloid P component,SAP)缺失对小鼠动脉粥样硬化形成的影响。方法构建载脂蛋白E(Apolipoprotein E)和SAP双基因敲除小鼠(ApoE-/-;SAP-/-)作为实验组,ApoE-/-小鼠作为对照组。2组小鼠各12只,组内雄雌小鼠各半。在小鼠第8周龄时,体质量约20 g,此时开始同时给予2组小鼠高脂饮食,于第16周龄时获取小鼠全血分离得到血清进行血脂分析,处死小鼠后获取其心脏,胸腹主动脉进行油红O染色并进行病理统计。结果双基因敲除小鼠组的高密度脂蛋白胆固醇(HDL-C)明显高于对照小鼠(P<0.01),低密度脂蛋白和高密度脂蛋白的比值和动脉粥样硬化指数(AI)小于对照组小鼠且差异显著(P<0.01),而总胆固醇(TC),甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)浓度无显著性差异(P>0.05);主动脉根部切片和胸腹主动脉的油红O染色结果表明SAP缺失明显减轻了小鼠动脉粥样硬化斑块面积(P<0.01)。结论 AP缺失可能通过上调HDL从而抑制ApoE-/-小鼠动脉粥样硬化斑块形成。Objective To observe the effect of serum amyloid P component （SAP） deficiency on ath erosclerosis （AS）in mice. Methods Apolipoprotein E （ApoE） and SAP knockout mice served as an experimental group （n = 12） and ApoE knockout mice served as a control group （n = 12）. Their average body weight was about 20 g at the age of 8 weeks. The animals were then fed with high fat diet and killed at the age of 16 weeks. Blood, heart, and thoracico-abdominal aorta tissue samples were taken. Serum lipid was analyzed and the AS lesion Plaque area was measured after oil red O staining. Results The HDL-C level was significantly higher while the LDL-C/HDL-C value and atherogenic index（ AI）were significantly lower in experimental group than in control group（ P 〈 0. 01 ）. However, no significant difference was observed in TC, TG and LDL-C levels between the two groups （ P 〉 0. 05 ）. Oil red O staining showed that SAP deficiency significantly reduced the area of atherosclerosis plaques in heart and thoracico-abdominal aorta tissue samples （ P 〈 0. 01 ）. Conclusion SAP deficiency inhibits formation of atherosclerosis plaques in mice by up-regulating their HDL-C level.

关 键 词：血清淀粉样P蛋白 高密度脂蛋白 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病]