广州市2010年1、2、3型登革病毒E基因进化分析  

Envelope gene evolution analysis on type 1, 2, 3 dengue virus in Guangzhou in 2010

作　　者：白志军[1] 和鹏[2] 狄飚[1] 鲁恩洁[1] 罗雷[1] 杨智聪[1] 王鸣[1] 王玉林[1] 

机构地区：[1]广州市疾病预防控制中心,510440 [2]中山大学中山医学院

出　　处：《中华传染病杂志》2012年第3期152-156,共5页Chinese Journal of Infectious Diseases

基　　金：国家科技重大专项（2009ZX10004-306、2009ZX10004-309）;广州市医药卫生科技重点项目（2008-ZDi-12）;广州市医药卫生科技项目（2009-YB-228）;广州市科技局资助项目（2009Ji-C161）

摘　　要：目的测定广州市2010年1、2、3型登革病毒的E基因序列，探讨其来源及基因型。方法收集广州市2010年85例登革热患者急性期血清，用C6／36细胞培养分离登革病毒，RT—PCR法扩增全长E基因，测定序列并绘制系统发育树，结合流行病学资料进行信息学分析。结果85份血清标本中分离到1型和3型登革病毒株各6株，2型登革病毒2株，测序获得E基因序列。进化分析发现，1型和3型登革病毒分别来自不同的亚型，即亚洲型和南太平洋型，印度次大陆型和东南亚／南太平洋型；2型登革病毒来自马来西亚／印度次大陆型。结论推测广州市2010年2型登革病毒为输入性。1型和3型登革病毒中各有4株为输入性，余各2株广州本地病例毒株尚须从蚊媒介来进一步证实其来源。Objective To analyze the Envelope （E） gene of type 1,2,3 dengue virus isolated from Guangzhou in 2010, and to investigate the infection source and virus genotypes. Methods Eighty-five serum samples were collected from 85 patients in acute phase of dengue fever. Dengue virus was cultured and isolated by C6/36 cells. The whole length of E gene was amplified by reverse transcriptase-polymerase chain reaction （RT-PCR） and then sequenced. The phylogenetic tree was drawn by neighbor-joining method. The bioinformatics analysis was performed by combining the phylogenetic information and the epidemiologic data. Results Six strains of type 1 dengue virus, two strains of type 2 dengue virus and six strains of type 3 dengue virus were isolated from 85 samples. The E gene sequence of these strains was obtained by sequencing. The phylogenetic analysis showed that type 1 and 3 dengue virus belonged to two genotypes （Asian and South Pacific ocean, India subcontinent and Southeast Asia/South Pacific ocean, respectively）, and type 2 dengue virus belonged to one genotype （Malaysia/India subcontinent）. Conclusion It＇s presumed that all strains of type 2 dengue virus are imported, four strains of type 1 dengue virus are imported and four strains of type 3 dengue virus are imported, the remaining two stains of type 1 and two stains of type 3 dengue virus need mosquito intermediary research further to prove their origins.

关 键 词：登革热病毒 病毒包膜蛋白质类 基因 病毒 序列分析 基因型 

分 类 号：R3[医药卫生—基础医学]