含神经生长因子壳聚糖神经导管修复大鼠坐骨神经10mm缺损  

Sciatic nerve repair across a 10-mm defect bridged by a chitosan conduit reinforced with nerve growth factor in rats

作　　者：顾剑辉[1] 王鸿奎[2] 胡文[2] 赵微加[2] 杨宇民[2] 

机构地区：[1]南通大学附属医院手外科 [2]南通大学神经再生重点实验室,江苏226001

出　　处：《交通医学》2012年第1期13-15,共3页Medical Journal of Communications

基　　金：国家自然科学基金资助项目(30970713);江苏省自然科学基金研究项目(BK2009158)

摘　　要：目的:研究京尼平交联的含神经生长因子的壳聚糖神经导管修复大鼠周围神经缺损的可行性。方法:利用含神经生长因子的壳聚糖神经导管桥接修复大鼠坐骨神经10mm缺损为实验组共5只,缺损组对照共5只。术后6个月行电生理学检测,并对再生神经组织和靶肌行形态学观察。结果:术后6个月,实验组术侧均能记录到复合肌动作电位,而缺损组术侧均未能记录到复合肌动作电位。抗NF-200免疫组化染色显示,实验组再生神经中段再生轴突分布密集。肌肉三色染色显示,实验组腓肠肌无明显萎缩,亦未见明显增生的胶原纤维;缺损组腓肠肌明显萎缩,同时可见大量胶原纤维增生。结论:术后6个月,京尼平交联含神经生长因子壳聚糖神经导管,能较好修复大鼠坐骨神经10mm缺损,实现靶肌神经功能的重支配。Objective： To study the repair outcome of chitosan nerve conduits immobilized with NGF by genipin crosslinking.Methods： Chitosan nerve conduits immobilized with NGF were used to bridge 10-mm sciatic nerve defects in rats（experiment group,n=5） and a non-grafted group as the negative control（gap group,n=5）.Six months after the surgery,electrophysiological assessments and morphological analyses were performed on both the regenerated nerves and the target muscles.Results： Six months after the surgery,compound muscle action potentials（CMAPs） were detected in all the animals of the experimental group but not in the gap group.Immunohistochemistry of NF-200 protein staining showed that regenerated nerve fibers were densely distributed in the mid-conduit in the experimental group.Masson trichrome staining revealed that the gastrocnemius muscle showed no remarkable atrophy and no obvious hyperplasia of collagen fibers in the experimental group in contrast to typical atrophy and hyperplasia of collagen fibers in the gap group.Conclusion： Six months after the surgery,the 10-mm sciatic nerve defect was well repaired by chitosan nerve conduits immobilized with NGF by genipin crosslinking.

关 键 词：坐骨神经缺损 京尼平 神经生长因子 壳聚糖 免疫组织化学法 大鼠 

分 类 号：Q819[生物学—生物工程]