儿童呼吸道感染流感嗜血杆菌的生物学分型及耐药性  

Biology grouping and drug resistance of Haemophilus influenzae from respiratory tract infection in children

作　　者：王运中[1] 季伟[2] 陶云珍[1] 吾金彪[1] 丁云芳[1] 

机构地区：[1]苏州大学附属儿童医院检验科,江苏省215003 [2]苏州大学附属儿童医院呼吸病科,江苏省215003

出　　处：《江苏医药》2012年第7期777-779,共3页Jiangsu Medical Journal

基　　金：苏州社会发展科技计划项目(SS0702)

摘　　要：目的了解苏州地区呼吸道感染患儿感染流感嗜血杆菌(HI)的生物型分布及耐药性。方法用负压吸取呼吸道分泌物进行培养,对HI进行生物学分型,头孢硝噻酚检测其β-内酰胺酶,并采用纸片法进行药敏试验。结果获得HI 154株,生物型以Ⅱ型和Ⅲ型为主,占分离总数的81.2%。Ⅱ、Ⅲ、Ⅶ型在各季节的检出率差异明显(P<0.05)。各型产β-内酰胺酶分别为Ⅰ型33.3%、Ⅱ型41.7%、Ⅲ型32.3%、Ⅶ型35.7%、Ⅷ型25.0%。HI对氨苄西林耐药率为35.7%,其多重耐药率为22.7%;且主要为产β-内酰胺酶株。结论苏州地区儿童感染HI以生物型Ⅱ型和Ⅲ型为主,且以Ⅱ型产酶率最高;HI耐药形势越来越严峻。Objective To investigate the distribution of biotype and drug resistance of Haemophilus influenzae（HI） from respiratory tract infection in children in Suzhou district,China.Methods Respiratory tract secretions for culture was obtained by vacuum suction and biology grouping was done for the isolation of HI.The beta-lactamase of HI was detected by nitrocefin and drug resistance was tested by Kirby-Bauer disc method.Results A total of 154 strains of HI was isolated,among which biotypes of Ⅱand Ⅲ were the two most common ones,and accounted for 81.2%.There was a significant season difference of the detection rate for biotypes of Ⅱ,Ⅲ and Ⅶ（P〈0.05）.The beta-lactamase positive rate was 33.3% for biotypeⅠ,41.7% for biotypeⅡ,32.3% for biotype Ⅲ,35.7% for biotype Ⅶ and 25.0% for biotype Ⅷ.The resistance rate of HI to ampicillin was 35.7% and the muti-resistance rate of HI was 22.7%,mainly producing beta-lactamase of HI.Conclusion The biotypes of Ⅱ and Ⅲ are the main common types in Suzhou district.The beta-lactamase positive rate of biotypeⅡ is the highest one.The situation of drug resistence of HI has been serious day by day.

关 键 词：流感嗜血杆菌 儿童 

分 类 号：R446[医药卫生—诊断学]