冷应激建立家兔动脉粥样硬化不稳定斑块模型的方法和评价  

Establishment and evaluation of rabbit unstable atherosclerotic plaque model induced by cold stress

作　　者：郑晓慧 高雪 李妍 刘丽文 易小敏 陈磊 赵永峰 宋宏萍 张荣庆 王海昌 

机构地区：[1]第四军医大学第一附属医院西京医院心脏内科,西安710032 [2]超声科 [3]泌尿外科 [4]第四军医大学第二附属医院唐都医院神经外科

出　　处：《中华老年医学杂志》2012年第4期330-334,共5页Chinese Journal of Geriatrics

基　　金：十一五军队指令性计划课题（10MA028）

摘　　要：目的探讨建立冷应激家兔腹主动脉粥样硬化不稳定斑块模型的方法和评价。方法60只雄性纯种新西兰大白兔，数字抽签随机分为3组（每组20只）。冷应激组高脂喂养加球囊损伤，此外除手术后1周每天置于4℃环境中给予冷应激刺激1h，持续到第20周；球囊损伤组单纯高脂喂养加球囊损伤；对照组普通颗粒饲料喂养无特殊处理。20周末观察血脂、炎性因子、斑块形态、斑块组成成分及新生血管形成的变化。结果冷应激组与球囊损伤组兔血脂比较，差异无统计学意义。冷应激组氧化型低密度脂蛋白（ox-LDL）、超敏C反应蛋白（hs-CRP）、白细胞介素（IL）-8分别为（56．14±14．3）mg／L、（149．1±78．3）mg／L、（97．6±17．9）／xg／L，高于球囊损伤组（42．9±13．8）rag／L、（94．5±57．3）mg／L、（57．5±18．3）ug／L，差异有统计学意义（t值分别为2．78、6．91、14．94，均P〈0，05）；冷应激组巨噬细胞含量为（30．9±5．6）％，高于球囊损伤组（18．7±4．8）％，差异有统计学意义（t=6．88，P〈0．05）；斑块内新生血管生成率冷应激组74．1％（23／31），高于球囊损伤组的20．0％（5／20），差异有统计学意义（X2=16．26，P〈0．05），对照组无斑块形成。结论冷应激结合高脂饮食加球囊损伤可成功建立兔动脉硬化不稳定斑块模型，其方法优于传统单纯高脂饮食加球囊损伤。Objective To establish and evaluate unstable atheroselerotic plaque model in abdominal aorta induced by cold stress. Methods Sixty male New Zealand white rabbits were randomly divided into three groups： cold-stress group fed with high fat diet and followed by balloon- induced arterial wall injury of abdominal aorta at week 2 and exposed to cold （4℃） for 1 h per day except for the first postoperative week, balloon-injury group treated by high fat diet plus balloon- injury, control group fed a normal chow without any treatment. Pathological changes of atherosclerotic plaques among these groups were evaluated at 20 weeks. Meanwhile, serum concentrations of blood lipid , oxidized low density protein（omLDL）, hypersensitive C-reaction protein （hs-CRP）and interleukin （IL）-8 were determined. Results There was no difference in blood lipid level between cold-stress and balloon-injury groups. Serum concentrations of ox-LDL〈（56.1± 14.3） mg/L vs. （42.9±13.8）mg/L], hs-CRP（（149.1±78.3）mg/L vs. （94.54±57.3）mg/L], IL-8 [（97.6± 17.9）ug/L vs. （57.5± 18.3）ug/L]and macrophage infihration[（30.9± 5.6）% vs. （18.7 1 4.8） %] were significantly higher in cold-stress group than in balloon-injury group （t = 2.78, 6.91, 14.94,6.88, all P〈0.05）. Higher angiogenesis rate of atherosclerotie plaque in cold-stress group （23/31,74. 1%） was observed in comparison with group balloon-injury（5/25,20.0%） （X2 = 16.26, P〈0.05）. Conclusions Establishment of rabbit unstable atherosclerotie plaque model induced by cold stress in synergy with high fat diet and balloon-injury is feasible, which is superior toconvenlional method through high fat diet plus balloon-injury surgery.

关 键 词：低温 应激 腹腔动脉 动脉硬化 新生血管化 病理性 

分 类 号：R543.5[医药卫生—心血管疾病] R-332[医药卫生—内科学]