广州无偿献血人群中丙型肝炎抗体阳性者HCV基因型与病毒载量的关系  

The correlation of HCV genotypes and viral loads among volunteer blood donors in Guangzhou

作　　者：廖峭[1] 许茹[2] 王敏[2] 黄珂[2] 熊华平[2] 叶欣[2] 戎霞[2] 罗广平[2] 付涌水[2] 王继红[1] 

机构地区：[1]重庆医科大学实验教学中心生物化学与分子生物学实验室,重庆400016 [2]广州血液中心,广州510095

出　　处：《中国免疫学杂志》2012年第3期242-245,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.30872162);广州市医药卫生重大项目(No.2009-ZDa-05);2010年广州市科技局重大科技专项(No.2010U1-E00661)

摘　　要：目的:了解最新广州地区无偿献血人群丙型肝炎病毒(HCV)基因型与病毒载量的关联性。方法:收集2008～2011年广州地区无偿献血人群中抗-HCV阳性标本605份,采用荧光定量PCR(Q-PCR)的方法对其进行核酸及病毒载量检测,阳性标本作NS5B基因扩增;核苷酸序列测定后运用DNASTAR、BioEdit和Mega4.0等软件作序列分析和基因分型,采用SPSS16.0软件对病毒载量与基因型(亚型)的关联性进行分析。结果:337份HCV RNA阳性的标本扩增出NS5B基因320份,HCV 1b、6a、3a、2a、3b、1a、6n比例依次为45.00%、33.44%、8.75%、7.81%、4.38%、0.31%和0.31%。HCV1b与2a、3a、6a、6a与2a、3a之间病毒载量存在显著差异:HCVba病毒载量高于2a、3a和6a,HCV6a病毒载量高于2a和3a。结论:广州地区无偿献血人群中HCV1b和6a为主要亚型且其病毒载量高于其他亚型。Objective：To determine the lastest correlation between HCV genotypes and viral loads among volunteer blood donors in Guangzhou.Methods：605 anti-HCV positive plasma samples were obtained from volunteer blood donors in Guangzhou during year of 2008 to 2011.Firstly,HCV RNA in those samples were detected by quantitative polymerase chain reaction（Q-PCR）.Futher,NS5B gene were detected by Q-PCR followed by direct DNA sequencing and phylogenetic analysis in HCV RNA positive samples.Results：337 samples were found HCV RNA positive in 605 anti-HCV positive plasma samples.320 samples（95%,320/337） were genotyped（based on NS5B gene） in 337 HCV RNA positive samples,and subtypes HCV 1b,6a,3a,2a,3b,1a,6n were detected at frequencies of 45.00%,33.44%,8.75%,7.81%,4.38%,0.31%,0.31%,respectively.HCV1b was associated with a significantly（P0.05） higher viral load compared to HCV6a,2a and 3a,while HCV6a also had a relatively higher viral load than HCV 2a and 3a.Conclusion：HCV 1b and 6a were the most predominant subtypes due to the higher viral load than the other subtypes among volunteer blood donors in Guangzhou.

关 键 词：丙肝病毒(HCV) 基因测序 基因分型 病毒载量 无偿献血者 

分 类 号：R512.63[医药卫生—内科学] R457.14[医药卫生—临床医学]