基于RAPD技术的韭菜品种间遗传多样性分析  

Analysis of Genetic Diversity in Leek Cultivars Based on RAPD

作　　者：李万昌[1] 乔保建 王俊伟[1] 余娇娇[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]平顶山市农业科学院,河南平顶山467001

出　　处：《河南农业科学》2012年第3期116-119,共4页Journal of Henan Agricultural Sciences

基　　金：河南省教育厅科技攻关项目(2010A210021)

摘　　要：为了研究韭菜种质资源的多样性和品种间的相似问题,利用RAPD分子标记技术分析了我国11个地区15个韭菜品种间的遗传多样性。结果表明,采用20条RAPD引物,共扩增出136个条带,其中多态性位点98个,平均多态性比率为72%。依据15个韭菜品种间的Nei’s遗传距离进行UPGMA聚类分析,在遗传距离为0.34处将15个韭菜品种分为4个类群:第1类群包含独根红、兰州白根和南京马鞭韭3个品种;第2类群包含汉中冬韭、上海强韭-3和上海强韭-6 3个品种;第3类群包含四季青、桂林小红根、天津大青苗、新疆白根和嘉兴雪莲5个品种;第4类群包含天津大金钩、环韭、平丰苔韭和抗寒薹韭4个品种。韭菜品种间遗传相似性和来源地之间没有必然的联系。Random amplified polymorphic DNA（RAPD） markers were used to estimate the genetic diversity in 15 cultivars of Allium tuberosum（leek） from 11 regions in China.Twenty random primers generated a total of 136 reproducible and discernible loci,among which 98 were polymorphic with the percent of polymorphic loci of 72%.Based on the Nei＇s genetic distances between the 15 cultivars,UPGMA cluster analysis was performed and showed that all cultivars were classified into four groups at genetic distance of 0.34,i.e.,Goup 1 contained 3 cultivars（Du Genhong,Lanzhou baigen and Nanjing mabianjiu）;Goup 2 included 3 cultivars（Hanzhong dongjiu,Shanghai qiangjiu-3 and Shanghai qiangjiu-6）;Goup 3 contained 5 cultivars（Sijiqing,Guilin xiaohonggen,Tianjin daqingmiao,Xinjiang baigen and Jiaxing xuelian）;Goup 4 included 4 cultivars（Tianjin dajingou,Huanjiu,Pingfeng taijiu and Kanghan taijiu）.There were no correlation between cultivars and origin regions of leeks.

关 键 词：韭菜 RAPD分子标记技术 遗传多样性 

分 类 号：S633.3[农业科学—蔬菜学]