右美沙芬对小鼠大脑5-羟色胺含量的影响  

Influences of Dextromethorphan on Monoamine Neurotransmitter 5-HT in Mouse Brain

作　　者：李迎春[1] 张久亮[2] 周莉红[1] 

机构地区：[1]武汉钢铁集团公司第二职工医院药剂科,武汉430085 [2]华中农业大学食品科技学院,武汉430070

出　　处：《医药导报》2012年第4期423-426,共4页Herald of Medicine

摘　　要：目的初步探讨右美沙芬通过增加小鼠脑部5-羟色胺(5-HT)的含量的镇咳机制。方法采用反相高效液相-荧光检测(RP-HPLC-FLD)法,以磷酸可待因为对照药,测定右美沙芬对小鼠脑部5种单胺类神经递质含量的影响。结果右美沙芬的镇咳机制与单胺类神经递质5-HT有关,右美沙芬组的5-HT含量比空白组增加了43.50%,差异有统计学意义(P<0.01),5-羟基吲哚乙酸(5-HIAA)含量比空白组增加35.00%,差异有统计学意义(P<0.05)。结论右美沙芬镇咳作用与小鼠脑部5-HT的释放有关。Objective To study the antitussive mechanism of dextromethorphan.Methods By using reversed-phase high-performance liquid chromatography（RP-HPLC） with fluorescent light detector,we detected the effect of dextromethorphan on the contents of five kinds of monamine neurotransmitters in mouse brain with codeine phosphate serving as a positive drug.Results The results indicated that the antitussive mechanism of dextromethorphan was concerned with monanine neurotransmitter 5-HT.In the dextromethorphan group,5-HT was 43.50% higher than in the blank group（P0.01）,and 5-HIAA was 35.00% higher than in the blank group（P0.05）.Dextromethorphan could increase the content of 5-HT in mouse brain to induce antitussive effects,while the antitussice effects of codeine phosphate had no relationship with serotonergic mechanisms.Conclusion The antitussive mechanism of dextromethorphan is related with the release of monanine neurotransmitter 5-HT in mouse brain.

关 键 词：右美沙芬 5-羟色胺 神经递质 镇咳 高效液相-荧光检测法 

分 类 号：R971.2[医药卫生—药品] R965[医药卫生—药学]