双J管生物被膜菌相关感染特点及耐药性分析  

Characteristic of biofilm-associated infections in double J tube and their drug resistance analysis

作　　者：董妍[1] 

机构地区：[1]南京市江北人民医院检验科,210048

出　　处：《检验医学与临床》2012年第7期783-785,共3页Laboratory Medicine and Clinic

摘　　要：目的筛选双J管置留患者的双J管感染生物被膜菌,了解其临床分布与耐药性,探讨生物被膜菌在模拟体内条件下对抗菌药物的耐药性。方法收集150例双J管相关尿路感染患者的双J管及尿液,筛选生物被膜菌,增殖培养。并对生物被膜阳性菌在普通水解酪蛋白(MH)培养基与泊洛沙姆(Poloxamer)培养基上的耐药性差异进行分析。结果 150例双J管置留患者双J管中筛选出生物被膜菌42株,阳性率28%。生物被膜菌在Poloxamer培养基和MH培养基上药敏结果差异有统计学意义(P<0.05),且前者耐药性更强。结论该院双J管感染的生物被膜菌在临床上以葡萄球菌属和肠球菌属为主,生物被膜菌在MH培养基上耐药性与在Poloxamer培养基上耐药性有明显差异,Poloxamer培养基可以较真实的表现生物被膜菌药物耐受情况,其耐受性更强。Objective To screen biofilm bacteria in the patients with indwelling double J tube to understand their clinical distrbution and drug resistance, and to investigate their resistance to antibiotics under simulating in vivo conditions. Methods Double J tube and urine were collected from 150 patients with double J tube associated urinary infections for screening biofilm bacteria and proliferation culture. The difference of drug resistance of positive strains of biofilm on Poloxamer medium and common MH medium was analyzed. Results Totally 42 strains of biofilm bac teria were screened out from double J tube in 150 cases of indwelling double J tube, showing the positive rate of 28 %. The results difference of the drug sensitivity test of biofilm bacteria in Poloxamer medium and MH medium was statistically different（P〈0.05）, and the former was stronger. Conclusion Biofilm bacteria infection in double J tube are mainly Staphylococci and Enterococci. The antibiotc resistance of biofilm in MH medium and Poloxamer medium is different. Poloxamer medium may display the true drug resistance of biofilm bacteria and has stronger tolerance.

关 键 词：尿路感染 双J管 生物被膜 耐药性 

分 类 号：R978.19[医药卫生—药品]