深静脉穿刺针病原菌分布情况及其药敏分析  

Distribution and drug resistance spectrum analysis of clinical pathogens isolated from deep venipuncture needles

作　　者：崔东岚 马均宝 凌步致 吴智刚 

机构地区：[1]广东省佛山巿第一人民医院检验科,528000

出　　处：《检验医学与临床》2012年第7期786-787,790,共3页Laboratory Medicine and Clinic

摘　　要：目的了解本院2008～2010年深静脉穿刺针感染病原菌的临床分布及其药敏特点,为临床合理使用抗生素提供依据。方法大多数分离细菌的鉴定和药敏试验利用VITEK-2全自动微生物分析仪,少数利用手工鉴定和K-B法。念珠菌利用显色平板分离,鉴定和药敏试验利用ATB半自动细菌分析仪。药敏数据分析用WHO-NET5.4软件。结果共收集标本1 953例,检出病原菌377株。分离病原菌数量以肿瘤内科108株(28.6%)、重症监护病房98株(26.0%)、肾内科85株(22.5%)为主,种类以葡萄球菌属、肠球菌属为主。革兰阳性球菌对万古霉素、利奈唑烷、替考拉宁、呋喃妥因敏感性较好。革兰阴性杆菌对美罗培南、亚胺培南、阿米卡星、头孢哌酮/舒巴坦敏感性较好。肺炎克雷伯菌和大肠埃希菌产超广谱β-内酰胺酶比例分别为41.9%和46.1%。结论应加强对深部穿刺针病原菌的分布及药敏监测,注意合理使用抗生素。Objective To investigate the distribution and drug resistance spectrum of clinical pathogens isola- ted from deep venipuncture needles from 2008 to 2010 to provide the basis for rational antibiotics use. Methods Most bacterial isolates were identified by VITEK-2 and a few were identified by manual method and K-B method. Candida isolates were identified by color-display plate and the drug sensitivity test was performed by ATB semiautomatic bac- terial analyzer. The data of drug sensitivity tests were analyzed by WHONET5.4 software. Results 377 pathogens were isolated from 1953 clinical specimens in three years, which were mainly from the departments of oncology（108 strains, 28.6%）, ICU （98 strains, 26.0%） and nephrology（85 strains,22.5%）. The common isolates were dominated by staphylococcus and enterococcus. Gram-positive cocci isolates had good sensitivity to vancomycin, linezolid, teicoplanin and nitrofurantoin. Gram-negative isolates had good sensitivity to imipenem, meropenem, cefoperazone/ sulbactam and amikacin. The rates of K. pneumoniae and E. coli producing ESBLs were 41.9 % and 46.1%, respectively. Conclusion It is necessary to monitor the distribution and drug resistance of clinical pathogens isolated from deep venipuncture needles in order to use antimicrobial agents rationally.

关 键 词：深静脉穿刺针 病原菌 耐药性 

分 类 号：R657.330.5[医药卫生—外科学]