HPLC法测定杜仲粗皮及杜仲中松脂醇二葡萄糖苷的含量  

Determination of pinoresinol diglucoside in Eucommiabark and Cortex Eucommiaeby HPLC

作　　者：吴慧敏[1] 孔军[2] 杜红岩[3] 李钦[1] 

机构地区：[1]河南大学中药研究所,河南开封475004 [2]河南大学学生处,河南开封475001 [3]中国林业科学研究院经济林研究开发中心,河南郑州450003

出　　处：《河南大学学报（医学版）》2012年第1期5-7,11,共4页Journal of Henan University:Medical Science

基　　金：国家林业公益性行业专项(201004029);国家"948"项目(2008-4-66);河南省科技攻关重点项目(102102310188);河南省教育厅自然科学基金资助项目(2008A360003)

摘　　要：目的建立HPLC法测定杜仲粗皮及杜仲中松脂醇二葡萄糖苷含量的方法。方法选用Diamonsil-C18色谱柱(5μm,150mm×4.6mm),甲醇-水(23∶77)为流动相,检测波长为277nm,流速为1mL/min。结果松脂醇二葡萄糖苷在0.496～4.960μg范围内呈线性关系良好,r=0.999 9。松脂醇二葡萄糖苷平均回收率为98.83%,RSD=1.25%。结论方法简便、准确、重复性好,可用于杜仲粗皮及杜仲中松脂醇二葡萄糖苷含量的测定。Objective To establish a method for determining pinoresinol diglucoside in Cortex Eucommiae and Eucommia bark by HPLC.Methods PDG was analysed by using Diamonsil Cls column （5μm, 150 mm× 4.6 mm ） with the mobile phase of methanol-water （23 ： 77） at the flow rate of 1 mL/min, and the detective wavelength was 277 nm. Results The calibration curve of Pinoresinol diglucoside was linear in the tangs of 0. 496-4. 960μg （ r = 0. 999 9）, the average recovery of pinoresinol diglucoside was 98.83% （ RSD = 1.25%）. Conclusion The method is simple, accurate and suitable for the determination of pinoresinol diglucoside in Cortex Eucommiae and Eucornmia bark.

关 键 词：松脂醇二葡萄糖苷 HPLC 杜仲 杜仲粗皮 

分 类 号：R284.1[医药卫生—中药学]