中缅边境恶性疟原虫pfmdr1基因多态性及pfmsp1等位基因分型研究  

pfmdr1 gene polymorphism and pfmspl gene typing of Plasmodium falciparum from China-Myanmar border

作　　者：王珊珊[1] 董莹[1,2] 邓艳[2] 王剑[2] 

机构地区：[1]大理学院病原与媒介生物研究所普洱分部,普洱665000 [2]云南省寄生虫病防治所,普洱665000

出　　处：《国际医学寄生虫病杂志》2012年第2期85-88,共4页International JOurnal of Medical Parasitic Diseases

摘　　要：目的分析中缅边境恶性疟原虫多抗药基因1（Plasmodium falciparum multidrugresist．ancel，pfmdr1）基因编码蛋白86位点多态性及裂殖子表面蛋白1（P．falciparu mmerozoite surface protein1gene，pfmsp1）等位基因的分型特征。方法采用chelex-100法提取DNA，PCR扩增pfmdr1基因编码蛋白86位点及pfmsp1基因第2区与第3区部分片段，并对其进行DNA测序，对扩增片段基因的结构、同源性进行分析。结果检钡4的50份血样中，49份成功扩增pfmdr1基因，49份在第25位氨基酸均发生缺失，且在第27—29位氨基酸由STA都突变为EYR，9份在第35位氨基酸缺失，第86位氨基酸均未发生点突变。50份恶性疟患者样本中有49份共扩增出72个pfmsp1基因片段，以MAD20型为主导型占93．88％，K1、R033型为次之，并存在不同基因株混合感染现象。结论中缅边境恶性疟原虫株pfmdr1基因第86位氨基酸点无突变，pfrrup1存在MAD20型、K1型和R033型3种等位基因型，以MAD20型为优势虫株。Objective To identify fragments polymorphism of the Plasmodium falciparum multidrug resistance 1 （pfmdr1） gene encoding protein at codon 86 and the genotype of P. falciparum merozoite surface protein 1 gene （pfrnspl） from China-Myanmar border. Methods Genomic DNA of P. falciparum was extracted by ehelex-100 method, then PCR was applied to amplify fragments of pfmdr1 gene including N86Y mutation and the Blocks 2 and 3 of pfmsp1. Results The pfmdr1 gene N86Y mutation was not detected in 49 PCR products from the total of 50 samples. All of the PCR products had gene deletion at position 25 and mutant allele （EYR） at positions 27-30. Moreover, the position 35 of pfmdr1 gene was deleted in 9 of 49 samples. From 48 of 50 blood samples of P. falciparum patients, 70 gene fragments of blocks 2 and 3 of the pfmspl were amplified, of which the MAD20-type allele was dominant （93.88%）, followed by KI-type and RO33-type alleles. Also, mixed infection phenomena of the different allelie type existed. Conclusion The pfmdr1 gene encoding protein at eodon 86 has no mutation and three principal allelic types ofpfrnspl gene （MAD20, K1 and RO33 type） exist in China-Myanmar border, and the MAD20-type is dominant.

关 键 词：恶性疟原虫 pfmsp1基因 pfmdr1基因 多态性 基因分型 

分 类 号：R978.61[医药卫生—药品]