碱性纤维素酶高产菌株的选育及产酶条件研究  

Study on the Screening and Optimized Condition of Alkaline-Cellulase Higher Production Strain

作　　者：卢金珍[1] 孙金龙 任俊[1] 熊汉国[3] 

机构地区：[1]武汉生物工程学院生物工程系,湖北武汉430415 [2]武汉马应龙药业股份有限公司 [3]华中农业大学食品科学技术学院

出　　处：《现代农业科技》2012年第6期40-41,共2页Modern Agricultural Science and Technology

摘　　要：以产碱性纤维素酶菌株H-1为出发菌株进行紫外线诱变,选育出一株产酶能力是出发菌株2.46倍且产酶性能稳定的高产菌株M-H-l。通过液体振荡培养法,对M-H-l的发酵培养基进行初步研究。结果表明:菌株M-H-l的最适碳源为麸皮,氮源为豆饼粉,无机盐为MgSO4,最适碳氮比为4∶1。The original strain H-1 was mutated by UV in the paper,and its enzyme productivity was 2.46 fold higher than that of parent strain.The mutant M-H-l was stable in the alkaline cellulase production.Through the liquid culture method,the fermentation conditions of M-H-l were studied.The results showed that the better carbon source was wheat bran,nitrogen source was soybean powder,salt was MgSO4,and the optimal carbon and nitrogen ratio was 4∶1.

关 键 词：菌株M-H-l 碱性纤维素酶 选育 产酶条件 

分 类 号：Q814[生物学—生物工程]