下肢慢性静脉功能不全血管壁炎性细胞浸润分布和定量研究  

Distribution and quantitative study of inflammatory cells infiltration in vessel wall of chronic venous insufficiency in lower limbs

作　　者：陈智年[1] 李凤臣[1] 王国华[1] 李泽宇[1] 张桂凌[1] 任洁[1] 

机构地区：[1]河南省新乡市中心医院普外二科,453000

出　　处：《中国实用医刊》2012年第8期7-9,共3页Chinese Journal of Practical Medicine

摘　　要：目的探讨淋巴细胞、单核一巨噬细胞和肥大细胞在血管壁中的浸润与下肢慢性静脉功能不全（chronicvenous insufficiency of lowlimbs，cvi）之间的关系。方法收集不同临床分期下肢慢性静脉功能不全的主干（中段）标本，C2-C3级20例，C4-C6级17例，对照组（正常静脉）7例。采用免疫组织化学染色LCA，CD68和甲苯胺蓝特殊染色分别检测淋巴细胞、单核。巨噬细胞和肥大细胞表达。结果C2～C3级、C4-C6级标本中单核-巨噬细胞表达与正常对照组差异有统计学意义（F：3．56，P〈0．05）；C2～C3级、c4～c6级标本中肥大细胞表达与正常对照组差异有统计学意义（F=5．13，P〈0．05）。结论单核-巨噬细胞和肥大细胞在血管壁的浸润可能是慢性静脉功能不全的一个重要病因。Objective To investigate the relationship between infiltration of lymphocytes, monocyte-macrophage and mast cells in vessel wall and chronic venous insu, fficiency of low limbs. Methods The bole specimens （midpiece） of chronic venous insufficiency in low limbs in different clinical stages were collected, 20 cases of C2 - C3, 17 cases of C4 - C6 and 7 cases of normal venous tissue. Immunohistochemical staining and toluidine bluestaining were used to detect the expression of lymphocyte, monoeyte-macrophage and mast cells. Results There were significant differences in the expression levels of monocyte-macrophage ceils between C2 - C3, C4 - C6 specimens and normal control group （ F = 3.56, P 〈 0.05 ） ; there were significant differences in the expression levels of mast cells between C2 - C3, C4 - C6 specimen and normal control group （ F = 5. 13, P 〈 0.05）. Conclusions Infiltration of monocyte-macrophages and mast cells in vessel wall could be an important cause of chronic venous insufficiency.

关 键 词：静脉功能不全 淋巴细胞 单核-巨噬细胞 肥大细胞 

分 类 号：R543.6[医药卫生—心血管疾病]