二穗短柄草叶绿体RNA编辑位点的预测及其鉴定  

Prediction and Identification of RNA Editing Sites in Chloroplast Transcripts of Brachypodium distachyon

作　　者：石文清[1,2] 邓平川[1] 李变丽[1,2] 牛善策[1] 聂小军[1] 王乐[1] Abdul.Wahid.Baloch 宋卫宁[1,2,3,4] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [4]国家小麦改良中心杨凌分中心,陕西杨凌712100

出　　处：《麦类作物学报》2012年第1期28-35,共8页Journal of Triticeae Crops

基　　金：教育部高等学校科技创新工程重大项目培育基金项目(707054)

摘　　要：RNA编辑是陆生植物叶绿体转录后基因表达调控的一种重要方式。为进一步探讨单子叶植物RNA编辑功能及其发生机制,本研究通过生物信息学方法,对二穗短柄草叶绿体的81个蛋白编码基因的RNA编辑位点进行了预测和鉴定分析,结果发现78个基因存在编辑位点,共检测到176个编辑位点,都是C到U的转换。其中ndhB最多,为14个编辑位点。为验证预测结果,利用blast工具比对了NCBI的二穗短柄草EST数据库,最终确定存在于17个基因中的34个编辑位点是真实存在的,其中19个为沉默编辑,15个为有效编辑。与5个不同单子叶禾本科物种18个蛋白编码基因的RNA编辑位点的比较发现,在rpoB-206位点,只有二穗短柄草发生了编辑,且只有ndhD-295(293)为部分编辑位点。RNA expression and Brachypodium editing is a way in which regulation of gene. In this distachyon were predicted chloroplast of terrestrial plant conduct post-transcriptional study, RNA editing sites of 81 and identified by bioinformatics chloroplast coding genes in methods. Finally we found 78 genes existed editing sites, 176 editing sites were detected and all of them were C to U conversion. Among them, ndhB contained 14 editing sites, as one of the genes possessing the most editing sites. Through BLAST analysis in the EST database of Brachypodium distachyon, 17 genes were proofed that having RNA editing sites, totally 34 editing sites were detected, of which 19 sites were silent edi ting, 15 sites were effective editing. Comparison on the editing sites of 18 chloroplast genes among five species of monocot grass, found that the editing in rpoB-206 site and partial editing in ndhD-295 （293） Site occurred only in Brachypodium distachyon.

关 键 词：二穗短柄草 叶绿体 RNA编辑位点 预测鉴定 

分 类 号：S512.9[农业科学—作物学] S330