固氮蓝藻与小麦共培养亲和藻株的筛选  

Screening of Affinity N2-fixing Cyanobacteria Strains on the Artificial Mixed Cultivation of Cyanobacteria and Wheat

作　　者：施军琼[1] 靳萍[1] 张帆[1] 赵昭[1] 张翔宇[1] 马剑敏[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《麦类作物学报》2012年第1期114-118,共5页Journal of Triticeae Crops

基　　金：河南省科技攻关项目（0624440039）;河南师范大学青年基金项目;河南省生物化学与分子生物学藿点学科资助项目.

摘　　要：为创建固氮蓝藻与小麦的共培养体系，用1株非固氮的自生蓝藻（聚球藻805）和20株固氮蓝藻（12株念珠藻、8株鱼腥藻）分别与小麦进行共培养并进行筛选，结果表明，与小麦共培养的念珠藻属（除藻株8981外）能使小麦的苗长、苗干重和根长显著增加。其中，藻株9101：1的效果最佳，小麦的苗长、苗干重、根长、根干重比加氮对照（＋N）分别增加了113．7％、110．3％、20．21％、28．16％，比缺氮对照分别增加了179．92％、310％、50．17％、80．13％；而鱼腥藻属只有藻株177能使小麦的苗长、苗干重、根长、根干重显著增加。进一步对各藻株的吸附能力进行比较，发现与12株念珠藻共培养的小麦根部的A665值显著大于与鱼腥藻共培养的小麦根部的A。值。通过观察蓝藻异形胞发现，含氮的BG11培养基能抑制异形胞的分化，而缺氮的BG11培养基和“缺氮BG11培养基＋小麦”处理则能显著增加异形胞的形成，且念珠藻的异形胞分化率显著高于鱼腥藻。与缺氮对照相比，在“缺氮BG11培养基＋小麦”处理中，念珠藻中只有9101：1的异形胞频率有差异，其异形胞分化率为9．47～16．55％。以上研究提示，念珠藻9101：1可以作为创建共培养体系的候选藻株。To establish the optimal artificial combination between N2-fixing eyanobacteria and wheat, a free-living cyanobacterium Synechococcus sp. 805 and 20 strains of N2-fixing eyanobacteria, including 12 Nostoc strains and 8 Anabaena strains, were tested in the present study. The results showed that many Nostoc strains （except the strains Nostoc sp. 8981） had significant effects on seedling height, seedling dry weight, and root length of wheat in comparison to the controls. Among all Nostoc strain, Nostoc sp. 9101 ： 1 was the best effective strain. Compared to the control （BGll medium）, the height, dry weight, root length, root dry weight of wheat seedlings inoculated with the Nostoc sp. 9101 ： 1 strain, increased by 113. 7%, 110. 3%, 20. 21% and 28. 16%, respectively. In addition, compared to the control （BGll medium without nitrogen）, the height, dry weight, root length, root dry weight of wheat seedlings increased by 179. 92%, 310%, 50. 17% and 80. 13%, respectively.ted that BG11 medium with nitrogen restricted the differentiation of cyanobacteria cells, but the nitrogen starvation and the treatment of wheat and BGll （N） medium could induce heterocyst development. However,only was theheterocyst frequency of the 9101 ： 1 strain in the treatment of wheat and BG11（-N） medium more obviously different than that in BGll （-N） medium. The heterocyst frequen- cy ranged from 9.45% to 16.55%. The results above suggested the Nostoc 9101 ： 1 may be a good candidate for the creation of co-cultivation system.

关 键 词：小麦 蓝藻 共培养体系 

分 类 号：S512.1[农业科学—作物学] S311