促红细胞生成素对兔心肌缺血/再灌注损伤保护作用的研究  

Protective effect of erythropoietin on myocardial ischemia/reperfusion injury in rabbits

作　　者：庞英[1] 马建群[2] 梁明亭[1] 

机构地区：[1]聊城市人民医院心内科,山东省252000 [2]滨州医学院附属医院心内科

出　　处：《中华临床医师杂志（电子版）》2012年第5期32-35,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的建立兔心肌缺血/再灌注损伤(I/R)模型,观察促红细胞生成素(EPO)对兔血清CK-MB浓度及心肌细胞凋亡的影响,探讨EPO对兔心肌I/R的保护作用及机制。方法新西兰大白兔16只,随机分为I/R组和EPO组。结扎兔左冠状动脉前降支制备兔I/R模型。EPO组于结扎左冠状动脉前降支的同时经耳缘静脉注入重组EPO,I/R组注入等量的生理盐水。检测指标:(1)分别于缺血前5 min、缺血60 min、再灌注60 min和再灌注180 min检测血清CK-MB浓度。(2)再灌注180 min时取缺血区心肌组织,检测凋亡指数。(3)制作心肌组织标本,观察心肌组织的病理变化。结果血清CK-MB浓度随缺血及再灌注时间的延长而增加(与前一时间点比较,P<0.01);缺血前EPO组与I/R组血清CK-MB浓度无统计学差异(P>0.05);缺血60 min、再灌注60 min、再灌注180 min时,EPO组血清CK-MB浓度均显著低于I/R组(均P<0.01)。与I/R组相比,EPO组的缺血区心肌细胞的凋亡指数显著下降(P<0.01)。结论 EPO对兔心肌I/R具有明显的保护作用,其机制可能与稳定心肌细胞膜结构,减少心肌酶的释放和抑制心肌细胞凋亡有关。Objective To investigate the influence of Erythropoietin on cardiac myocyte apoptosis and CK-MB level in myocardial ischemia/reperfusion（I/R）injury of rabbit,and to explore the protective effects of Erythropoietin and its mechanisms.Methods Sixteen New Zealand rabbits were randomly divided into two groups：I/R injury group（C）and Erythropoietin group（EPO group）.Rabbits were subjected to descending coronary artery（LAD）occlusion to establish rabbit myocardial I/R injury models.When the LAD was ligated,recombinant human Erythropoietin（rhEPO）was injected in EPO group by ear vein,I/R group were injected saline equivalently.Observinvg indexs：（1）testing the serum concentration of CK-MB at 5 min before occlusion,60 min after occlusion,60 min and 180 min after reperfusion.（2）testing the apoptotic index of the corresponding ischemic myocardium myocardial cell at 180 min after reperfusion,observing the general myocardial tissue morphosis organizational structure by light microscope.Results The serum CK-MB concentration increased gradually with the ischemia and reperfusion time（P〈0.01）.Before ischemia,the serum CK-MB concentrations had no significant difference between EPO group and the I/R group（P〈0.05）.The CK-MB concentrations in EPO group were markedly lower comparing with the I/RI group at 60 min after ischemia,60 min and 180 min after reperfusion（all P〈0.01）.Compared with the I/R group,the apoptotic index of ischemic zone in EPO group decreased significantly（P〈0.01）.Conclusions EPO has clear protective effects on the myocardial I/R injury in rabbits.The mechanisms of its cardioprotection may be associated with stabilizing the myocardium cell membrane structure to decrease the release of myocardial enzyme and to inhibit cardiac myocyte apoptosis

关 键 词：红细胞生成素 再灌注损伤 细胞凋亡 肌酸激酶 MB型 

分 类 号：R541.4[医药卫生—心血管疾病]