萎缩性胃炎胃黏膜组织不同亚型肠上皮化生中突变型p53和C-myc的表达  

The expressions of mtp53 and C-myc proteins in different subtypes of intestinal metaplasia

作　　者：黄珏[1] 赖跃兴 杨道华 李清华[2,4] 徐萍[2,3] 

机构地区：[1]南京医科大学,在读研究生210029 [2]南京医科大学附属上海松江中心医院 [3]上海交通大学附属第一人民医院松江分院消化科 [4]上海交通大学附属第一人民医院松江分院病理科

出　　处：《中华临床医师杂志（电子版）》2012年第6期32-34,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：上海市卫生局课题(2009251);上海市科委医学引导类科技项目(114119b0100)

摘　　要：目的探讨萎缩性胃炎伴不同亚型肠上皮化生胃黏膜组织中突变型p53(mtp53)和C-myc蛋白的表达及意义。方法通过AB-PAS和HID-AB黏液染色区分肠上皮化生亚型,应用免疫组织化学Elivision法检测各组mtp53和C-myc蛋白的表达。结果不完全性结肠化生、不完全性小肠化生、完全性结肠化生、完全性小肠化生和非萎缩性胃炎胃黏膜中mtp53表达率分别为:25.00%、4.25%、5.88%、6.25%、0.00%,C-myc的表达率分别为:57.78%、23.40%、17.65%、25.00%、10.00%,不完全性结肠化生mtp53蛋白表达高于非萎缩性胃炎(P<0.01);不完全性结肠化生C-myc蛋白表达高于其他亚型肠化生和非萎缩性胃炎(P<0.05)。结论 mtp53蛋白和C-myc蛋白过表达可能参与了胃黏膜肠化及癌变的过程,同时提示不完全性结肠化生可能与胃癌的发生关系密切。Objective To investigate expressions and significance of mutant p53（mtp53）and C-myc proteins in different subtypes of intestinal metaplasia in companion with atrophic gastritis.Methods Intestinal metaplasia cases were classified into different subtypes by Alcian blue/periodic acid Schiff（AB-PAS）and high iron diamine/Alcian blue（HID-AB）mucinous staining method.Immunohistochemical Elivision methods was used to determine the expressions of mtp53 and C-myc proteins.Results The positive rates of mtp53 protein in incomplete colonic type of intestinal metaplasia,incomplete small intestinal type,complete colonic type and complete small intestinal type was 25.00%,4.25%,5.88%,6.25%,0.00%,and the positive rates of C-myc protein was 57.78%,23.40%,17.65%,25.00%,10.00%.Expression of mtp53 protein in incomplete colonic type of intestinal metaplasia was higher than non-atrophic gastritis（P0.01）.C-myc protein in incomplete colonic type of intestinal metaplasia was higher than other subtypes of intestinal metaplasia or non-atrophic gastritis（P0.05）.Conclusions Mtp53 and C-myc proteins may play an important role in intestinal metaplasia and gastric carcinoma,while incomplete colonic type of intestinal metaplasia may has close relationship with incidence of gastric carcinoma.

关 键 词：胃炎 萎缩性 肿瘤抑制蛋白质P53 原癌基因蛋白质C-MYC 肠上皮化生 

分 类 号：R735.2[医药卫生—肿瘤] R573.32[医药卫生—临床医学]