Effects of Bushen Huoxue Yin(补肾活血饮) on brain NF-κB and NO content in the parkinson's disease model mouse  

作　　者：李绍旦 刘毅 杨明会 

机构地区：[1]Institute of Traditional Chinese Medicine,PLA General Hospital

出　　处：《Journal of Traditional Chinese Medicine》2012年第1期67-70,共4页中医杂志（英文版）

基　　金：Supported by National Natural Science Foundation (No.30672762)

摘　　要：OBJECTIVE:To observe the effects of Bushen Huoxue Yin(补肾活血饮,BSHXY) on nuclear transcription factor kappa B(NF-κB) and nitric oxide(NO) in the brain of the Parkinson's disease(PD) model mouse.METHODS:Forty-five C57BL/6 mice were randomly divided into three groups;normal,model and BSHXY treatment groups.Concentrations of NF-κB and NO in mouse brain tissue were determined by ELISA and spectrophotometry,respectively.RESULTS:NF-κB concentration in brain tissue in the model group was 14.04±4.38 μg· L-1,which was higher than that in normal(P<0.01) and BSHXY(P< 0.05) groups.NO content in brain tissue in the model group was 5.93±0.79 μmol · gprot-1,which was also higher than that in model(P<0.01) and BSHXY(P<0.01) groups.However,there were no significant differences in the content of NF-κB and NO between BSHXY and normal groups(P>0.05).CONCLUSION:The mechanism of BSHXY for treatment of PD is possibly related to inhibition ofNF-κB activation and decreased NO content in the brain.OBJECTIVE：To observe the effects of Bushen Huoxue Yin（补肾活血饮,BSHXY） on nuclear transcription factor kappa B（NF-κB） and nitric oxide（NO） in the brain of the Parkinson＇s disease（PD） model mouse.METHODS：Forty-five C57BL/6 mice were randomly divided into three groups;normal,model and BSHXY treatment groups.Concentrations of NF-κB and NO in mouse brain tissue were determined by ELISA and spectrophotometry,respectively.RESULTS：NF-κB concentration in brain tissue in the model group was 14.04±4.38 μg · L-1,which was higher than that in normal（P0.01） and BSHXY（P 0.05） groups.NO content in brain tissue in the model group was 5.93±0.79 μmol · gprot-1,which was also higher than that in model（P0.01） and BSHXY（P0.01） groups.However,there were no significant differences in the content of NF-κB and NO between BSHXY and normal groups（P0.05）.CONCLUSION：The mechanism of BSHXY for treatment of PD is possibly related to inhibition ofNF-κB activation and decreased NO content in the brain.

关 键 词：Parkinson＇s disease Bushen Huoxue Yin Therapy ofTraditional Chinese Medicine Nuclear transcription factor kappa B Nitric oxide 

分 类 号：R285[医药卫生—中药学]