Study on effects of Zhi Zi(Fructus gardeniae) on non-alcoholic fatty liver disease in the rat  

作　　者：陈少东 李晶 周海虹 林曼婷 刘益华 张玉梅 赵正晓 李国辉 柳进 

机构地区：[1]TCM Department,Medical College of Xiamen University,Xiamen

出　　处：《Journal of Traditional Chinese Medicine》2012年第1期82-86,共5页中医杂志（英文版）

基　　金：Supported by Xiamen City Key Science and Technique Plan,NO.3502Z20100006;Xiamen University's Student Experimental Plan for New Ideas,NO.XDDC 2011090

摘　　要：OBJECTIVE:To investigate the effects of Zhi Zi(Fructus Gardeniae) on non-alcoholic fatty liver disease(NAFLD) induced by a high-fat diet in the rat.METHODS:A rat model of NAFLD was established using a high-fat diet.Twenty one rats were randomly divided into a normal group,a model group and a Zhi Zi treatment group,7 rats per group.Drinking water and the drug were intragastrically administrated for 5 weeks.Samples were then taken to observe pathological changes of the liver tissue(HE staining);changes in the fat metabolism pathway e.g.triglyceride(TG) and free fatty acid(FFA) content;alterations in liver function,i.e.serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activity;and differences in tumor necrosis factor α(TNF-α) and P-IkB protein expression in the liver tissue.RESULTS:Fatty degeneration and vacuole-like changes of different degrees occurred in hepatic cells of the model group.Markers for fat metabolism,serum ALT and AST activities,and expressionof TNF-α and P-IkB proteins in liver tissue significantly increased.Fat metabolism in the Zhi Zi group significantly reduced,as shown by a drop in marker levels.Serum ALT and AST activities,and expression of TNF-α,P-IkB proteins in liver tissue were also significantly decreased in this group.CONCLUSION:Zhi Zi has a very strong inhibitory action on lipidosis and inflammatory injury in the rat model of NAFLD.This mechanism may possibly be related to the inhibition of the free fatty acid metabolism pathway.OBJECTIVE：To investigate the effects of Zhi Zi（Fructus Gardeniae） on non-alcoholic fatty liver disease（NAFLD） induced by a high-fat diet in the rat.METHODS：A rat model of NAFLD was established using a high-fat diet.Twenty one rats were randomly divided into a normal group,a model group and a Zhi Zi treatment group,7 rats per group.Drinking water and the drug were intragastrically administrated for 5 weeks.Samples were then taken to observe pathological changes of the liver tissue（HE staining）;changes in the fat metabolism pathway e.g.triglyceride（TG） and free fatty acid（FFA） content;alterations in liver function,i.e.serum alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activity;and differences in tumor necrosis factor α（TNF-α） and P-IkB protein expression in the liver tissue.RESULTS：Fatty degeneration and vacuole-like changes of different degrees occurred in hepatic cells of the model group.Markers for fat metabolism,serum ALT and AST activities,and expressionof TNF-α and P-IkB proteins in liver tissue significantly increased.Fat metabolism in the Zhi Zi group significantly reduced,as shown by a drop in marker levels.Serum ALT and AST activities,and expression of TNF-α,P-IkB proteins in liver tissue were also significantly decreased in this group.CONCLUSION：Zhi Zi has a very strong inhibitory action on lipidosis and inflammatory injury in the rat model of NAFLD.This mechanism may possibly be related to the inhibition of the free fatty acid metabolism pathway.

关 键 词：Zhi zi（Fructus Gardeniae） Non-alcoholic liver disease LIPIDOSIS Inflammatory injury Free fatty acid metabolism pathway 

分 类 号：R285[医药卫生—中药学]