氧因素对趋磁螺菌AMB-1磁小体形成及其相关基因表达的影响  

Effects of Oxygentension on Magnetosome Formation and Expression of mms13,mms6 andmagA in Magenetospirillum Magneticum AMB-1

作　　者：陈小丽[1] 纪国强[1] 孔登[1] 付新华[1] 陈永[1] 邢秋翠[1] 王小柯[1] 

机构地区：[1]潍坊医学院生物化学与分子生物学教研室,山东潍坊261053

出　　处：《潍坊医学院学报》2012年第1期21-25,共5页Acta Academiae Medicinae Weifang

基　　金：山东省科技攻关计划资助课题（课题编号：2009GG10002079）

摘　　要：目的 观察氧因素对趋磁螺菌AMB-1生长量、磁小体形成及其相关基因表达的影响,为确定磁小体合成最适条件和研究磁小体形成机制提供实验依据.方法 在地磁有铁条件下,AMB-1经培养驯化接种,分别置于好氧和微好氧条件下培养.定时取样检测菌液OD600和Cmag值来研究细胞生长量和磁性增长量;用透射电镜观察16h菌样的磁小体形态;qRT-PCR检测磁小体相关基因mms13,magA和mms6的表达.结果 有铁微氧种子转至好氧条件培养12h后,其OD600值较对照组显著升高,2.5h后Cmag值明显降低,磁小体链短而疏松.16h菌样mms13,magA,mms6基因表达无明显差异.有铁好氧种子转至微氧条件培养14h后,其OD600值较对照组显著降低,10h后Cmag值显著增高,磁小体呈紧密的长链分布,16h菌样mms13基因表达量增加6倍,magA和mms6明显增高.结论 好氧条件有利于AMB-1细胞的生长,微氧条件促进磁小体的形成,并明显影响mms13,magA和mms6基因表达.Objective To invesligale effects of oxygenlension on magnetosome formation and expression of nmlsl3,mms6 and magA in Magenetospirilhtm magneticum AMB-I. 11 can provide some basis fl^r determing the optimum magnetosome synthesis conditions and studing magnetosome fin＇marion mechanism. Methods Under geomagnetic iron condition,the trained AMB-I were inoculated Io EMS（,M and cuhivated in micro-aerobic and aerobic ~,onditions, respecliwdy. To research the effects of oxygentension on growth and magneh^some forma- tion,regularly lest （）l）t,~~ and C,,,,,~ values, and ohserve the ~＇ell witll transmission electron microscope. The expression of mm^d3, mm^6 and magA which were related to magnelosome formation by qRT-PCR were analyzed. Results In aerobiccondition,OI）e^x~ increased rapidly after twelve hours and C,,,,,~ value had a obvious drop after 2.5 hours,at 161h the expression of mms l3 ,m.gA and rams6 had no signifi~.ant difl^r- ence. Magneloso,ne chain was short and loose. In micro-aerobic condition,OD600 increased significantly from Ihe 14lh hour and C xalue re- duced obviously after 10 hours,at the 16/h hour the expression of mms l3 increased 6 times,magA and mm.d6 increased signifintly. Magnetosome shaped in loselv long-chain. Conclusion Aerobic condition can facilitate the cell growth,micro-aerobic promote maguetosome firmation and effect the expression of mms l3,magA and mm.~6 in Magenet.spirilhml magnetivum A MB -1.

关 键 词：趋磁螺菌AMB-1 氧因素 磁小体 基因表达 

分 类 号：R34[医药卫生—基础医学]