美洲大蠊提取液对UC大鼠模型IL-1β,TGF-β1表达的影响  

Effects of America Cockroach Extract on Expression of TGF-β1 and IL-1β in Rats Model with Ulcerative Colitis

作　　者：白秀慧[1] 原皓[1] 卢燕[1] 何小姣[1] 王鹤鸣[1] 赵雪曼[1] 

机构地区：[1]潍坊医学院内科学教研室,山东潍坊261053

出　　处：《潍坊医学院学报》2012年第1期63-66,共4页Acta Academiae Medicinae Weifang

摘　　要：目的 建立大鼠溃疡性结肠炎模型,观察美洲大蠊提取液（ACE）对大鼠溃疡性结肠炎的治疗作用及对IL-1β、转化生长因子-β1（TGF-β1）产生和表达的影响.方法 健康雄性Wistar大鼠36只随机分为正常对照组（10只）与TNBS组（26只）.TNBS组用2,4,6-三硝基苯磺酸（TNBS）/乙醇灌肠建模,造模第3天,随机处死TNBS组大鼠6只观察造模效果.将TNBS组剩余20只大鼠随机分为模型对照组和ACE组,每组10只,ACE组给予ACE干预治疗10d,观察疾病活动指数（DAI）、结肠大体形态损伤和组织学变化;免疫组化法检测鼠结肠黏膜IL-1β,TGF-β1的表达情况,实时荧光定量技术检测结肠黏膜IL-1β及TGF-β1 mRNA的表达水平.结果 与模型对照组相比,ACE组DAI、结肠大体形态、组织学损伤显著改善（P〈0.05）;与模型对照组相比,ACE组IL-1β水平显著降低（P〈0.05）;与正常对照组相比,ACE组和模型对照组TGF-β1表达显著增高,而ACE组TGF-β1表达低于模型对照组,均有统计学意义（P均〈0.05）.结论 ACE对大鼠溃疡性结肠炎有疗效;TGF-β1参与了UC炎症损伤及转归过程,ACE通过降低IL-1β和TGF-β1的产生和表达可能是其治疗机制之一.Objective To explore the regulalory effects of America Cockroach Fxtract on interleukin-1β（ IL-1β） and Transforruing grow the factor-β 1（ TGF-β1 ） in rats model with ulcerative colitis （UC）. Methods Thirty-six heahhy-male Wista rats were randomized into normal control group（10） and TNBS group （26）. Ulcerative colitis was induced in the TNBS group by rectal administration of trinitrobenzene sulphonic acid（TNBS）. After three days,six rats in TNBS group were killed to observe mucosal lesion area. Then the twenty rats in TNBS group were randomized inlo model group and ACE group. The rats in ACE group were treated with ACE. Colon mueosal inflammation and dmuage were assessed by measuring disease aetivity index（DAI） mucosal lesion area and hislologieal damage scores. Levels of IL-1β and TGF-β1 were detected with immunohistochemistry. And the expression of IL-1β and TGF-β1 mRNA were detected hy real-time fluorescent quantitative polymerase chain reaction. Results Compared with the model group,the DAl,mucosal lesion area and histological damage scores in ACE group were significantly improved（P 〈 0.05）. Compared with the model group,the levels of IL-1β in ACE group were significantly deereased（ P 〈0.05） ,compared with the normal control gToup,the expression of TGF-β1 in ACE group and model group were significantly up-regulated and the expression of TGF-β1 in ACE group was lower than that in the model group respectively（ P 〈 0.05 ）. Conclusion ACE Ires therapeulie effect on UC. TGF-β1 may participate UC inflammatory and mucosal repair, ACE can down-regulate the levels of IL-1β and the expression of TGF-β1 ,which is the major mechanism of action in the treatment of ulcerative colitis.

关 键 词：结肠炎 溃疡性 美洲大蠊提取液 白细胞介素1Β 转化生长因子-Β1 

分 类 号：R547.62[医药卫生—心血管疾病]