宜兴百合组培快繁技术的研究  

Study on Tissue Culture Technology of Lilium lancifolium Thunb

作　　者：潘理云[1] 张海洋[1] 

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000

出　　处：《安徽农业科学》2012年第10期5748-5750,共3页Journal of Anhui Agricultural Sciences

基　　金：2011年浙江省大学生科技创新活动计划(新苗人才计划)资助项目(2011R425003)

摘　　要：[目的]采用组织培养的方法对宜兴百合(Lilium lancifoium Thumb)进行扩大繁殖研究。[方法]以宜兴百合鳞茎为外植体,在其不同的组织培养阶段采用不同的激素配比,观察培养效果,以建立宜兴百合的组培快繁体系。[结果]宜兴百合鳞茎不定芽诱导的最佳培养基为MS+3.0 mg/L 6-BA+0.5 mg/L NAA,继代培养的最佳培养基为MS+3.0 mg/L 6-BA+0.1 mg/L NAA,生根培养的最佳培养基为1/2MS+0.5 mg/L IBA。[结论]为提高宜兴百合的繁殖系数和加快其产业化生产提供技术支撑。[Objective] The aim was to use tissue culture method for the increased reproduction of Lilium lancifoium Thumb.[Method] Using bulbs of Lilium lancifoium Thumb as explants,observing the culture effect in its different tissue culture stages with different hormone combinations,to build Lilium lancifoium Thumb tissue culture system.[Result] The best adventitious bud induction medium of Lilium lancifoium Thumb bulbs was MS＋3.0 mg/L 6-BA＋0.5 mg/L NAA,the best subculture medium was MS＋3.0 mg/L 6-BA＋0.1 mg/L NAA,the best root induction medium was 1/2MS＋0.5 mg/L IBA.[Conclusion] It provided technical support for improving the propagation coefficient of Lilium lancifoium Thumb and speeding up its industrial production.

关 键 词：宜兴百合 组培 快繁 激素 

分 类 号：S644.1[农业科学—蔬菜学]