东北矮紫杉细胞悬浮培养中褐化问题的研究  

Research on the Browning in Suspension Cell Culture of Taxus cuspidata

作　　者：王培忠[1] 赵欣[1] 张宗申[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《安徽农业科学》2012年第10期5760-5762,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31070164);大连市青年科技人才基金(2006J23JH031)

摘　　要：[目的]研究东北矮紫杉悬浮培养细胞褐化现象,为紫衫细胞悬浮培养提供指导方法。[方法]以东北矮紫杉愈伤组织为试验材料,探讨不同培养基、N/P比、pH、培养转速、光照及强度等因素对矮紫杉细胞悬浮培养褐化的影响。[结果]将无褐化的愈伤组织转接到pH 7.0的2mB5培养基中,于22℃、1 500 lx光强、90 r/min转速的条件下光照培养24 h,细胞褐化现象能得到明显抑制。[结论]该研究结果为紫衫细胞悬浮培养奠定了基础,对于大规模工业化生产紫杉醇具有重要意义。[ Objective ] This study aimed to investigate the browning of T. euspidata cells in suspension culture and provide a guidance for the cell suspension culture of T. cuspidata. [ Method] T. cuspidata callus were used as experimental materials, to explore the effects of different medium, N/P ratio, pH,shaking speed,illumination time and light intensity and other factors on browning of T. cuspidata cells in suspension culture. [ Result] Non-browning callus were transferred to 2MB5 medium （pH 7.0） for light cuhure at 25 ℃ under hght intensity of 1 500 lx with shaking speed of 90 r/min for 24 h. Results showed that the cell browning was significantly inhibited. [ Conclusion] This study laid the foundation for cell suspension culture of T. cuspidata and had important significance to the large-scale industrial production of paclitaxel.

关 键 词：东北矮紫杉 褐化 愈伤组织 悬浮细胞培养 

分 类 号：S718.43[农业科学—林学]