SDS-聚丙烯酰胺凝胶电泳4种染色方法的比较研究  

A Comparative Study on Four Staining Methods for Antitumor Active Fraction of Periplaneta Americana

作　　者：吴少辉[1] 巫秀美[1] 张成桂[1] 董光平[1] 刘光明[1] 

机构地区：[1]大理学院药学院,云南大理671000

出　　处：《安徽农业科学》2012年第10期5763-5764,5770,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30560181);云南省重点产业创新工程基金项目(2008IF012)

摘　　要：[目的]比较SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)中蛋白质的染色方法,建立美洲大蠊抗肿瘤活性部位SDS-PAGE后更为合适的染色方法。[方法]以牛血清白蛋白(BSA)为标准品,采用考马斯亮蓝染色法、氯化钾染色法、钙染色法和银染色法进行比较,在此基础上,将样品美洲大蠊抗肿瘤活性部位进行SDS-PAGE电泳和染色。[结果]结果表明银染法能够准确、快速、简便的对美洲大蠊抗肿瘤活性部位的SDS-PAGE进行染色。[结论]为美洲大蠊药用价值的研究奠定了基础。[Objective] This study aimed to compare four staining methods for proteins of SDS-polyacrylamide gel electrophoresis（SDS-PAGE） and explore a suitable staining method for the antitumor active fraction of P.americana after polyacrylamide gel electrophoresis.[Method] BSA was used as the standard for the comparison of Coomassie brilliant blue staining method,potassium staining method,calcium staining method and silver staining method,on the basis,antitumor active fraction samples of P.americana were used for SDS-PAGE electrophoresis and staining.[Result] The results showed that silver staining method could be accurately,quickly and easily used for SDS-PAGE staining of the antitumor active fraction of P.americana.[Conclusion] This study had laid the foundation for exploring the medicinal value of P.americana.

关 键 词：SDS-聚丙烯酰胺凝胶 牛血清白蛋白 考马斯亮蓝染色法 

分 类 号：S312[农业科学—作物栽培与耕作技术] R931.6[农业科学—农艺学]