大肠杆菌glyA工程菌抗噬菌体菌株选育  

Selection of Phage-resistant Strains from Escherichia coli glyA Genetic Engineering Bacteria

作　　者：梅运军[1] 刘红 

机构地区：[1]武汉工业学院化学与环境工程学院,湖北武汉430023 [2]三峡高中,湖北宜昌443100

出　　处：《安徽农业科学》2012年第10期5771-5772,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]该研究旨在选育具有噬菌体抗性的大肠杆菌菌株。[方法]利用噬菌体诱发及紫外耦合噬菌体诱发筛选大肠杆菌glyA工程菌的抗噬菌体菌株。[结果]从噬菌体诱发筛选到的24株抗性菌株中筛选出了20株具有稳定抗性的抗性菌株,但其中仅有1株生长势优于原始株,酶活均低于原始株;耦合法诱导得到的41株中有39株具有较好的稳定性,其中7株生长势优于原始株,2株酶活大于原始株。[结论]利用紫外耦合噬菌体诱发筛选大肠杆菌glyA工程菌的抗噬菌体菌株能得到较好的试验效果。[Objective] This study aimed to select E.coli strains with phage-resistance.[Method] Phage-resistant strains of E.coli glyA genetic engineering bacteria were selected by phage induction and UV-coupling phage induction.[Result] By phage induction,20 strains with stable resistance were selected from the 24 phage-resistant strains,only one strain showed better growth condition than the original strains,but the enzymatic activities of the 20 strains all were lower than the original strains;41 phage-resistant strains were selected by UV-coupling phage induction,39 strains of which had better stability,including 7 strains that showed better growth condition than the original strains and two strains had higher enzymatic activities than the original strains.[Conclusion] UV-coupling phage induction is a suitable method to select phage-resistant strains from E.coli genetic engineering bacteria.

关 键 词：大肠杆菌 噬菌体 抗噬菌体 选育 

分 类 号：S188[农业科学—农业基础科学] Q939.97[生物学—微生物学]