产甲烷细菌保藏方法研究  

Study on the Preservation Method for Methanogenic Bacteria

作　　者：张万芹 康冀川[2] 王开功[3] 

机构地区：[1]兴以民族师范学院,贵州兴义562400 [2]贵州大学贵州省生化工程中心,贵州贵阳550025 [3]贵州大学动物科学学院,贵州贵阳550025

出　　处：《安徽农业科学》2012年第10期5775-5775,5778,共2页Journal of Anhui Agricultural Sciences

基　　金：贵州省教育厅自然科学研究资助项目(20090100)

摘　　要：[目的]研究适合产甲烷细菌保存的方法。[方法]采用严格厌氧技术,对从贵阳周边沼气池中初步筛选的28株产甲烷细菌进行保存,经过短期、长期保存方法试验,测得短期保藏6个月和12个月后,菌株存活率为71.4%,18个月为67%;长期保藏18个月菌株存活率为57.1%。[结论]结果为重新开展产甲烷细菌保藏方法的研究奠定了基础。[Objective] The aim was to study the preservation method for methanogenic bacteria.[Method] 28 strains of methanogenic bacteria which preliminarily screened from Guiyang peripheral methane tank were preserved.[Result] The results showed that the survival rates were 71.4% after 6 and 12 months,67% after 18 months for short-term preservation;after 18 months for long-term preservation,the survival rate was 57.1%.[Conclusion] The results lay the foundation for restudying the preservation method for methanogenic bacteria.

关 键 词：产甲烷细菌 保藏方法 存活率 

分 类 号：S-3[农业科学] Q939.96[生物学—微生物学]