野油菜黄单胞菌中gumD基因的过表达对产黄原胶的影响  

Effect of by overexpressing gumD in Xanthomonas campestris on the xanthan gum

作　　者：王桂兰[1,2] 张晓元[2] 陈晓燕[2] 朱希强[1,2] 凌沛学[1,2] 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东省生物药物研究院博士后科研工作站,山东济南250101

出　　处：《中国生化药物杂志》2012年第2期106-109,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家"十一五"科技支撑计划(编号:2008BAI63B08)

摘　　要：目的在野油菜黄单胞菌58(Xc58)中过量表达产胶基因gumD,提高黄原胶发酵产量和质量。方法通过PCR扩增、重组质粒构建、三亲本接合等方法,将重组质粒pBBR-gumD转入原始菌Xc58。结果工程菌与原始菌相比,黄原胶产量提高11.19%,黏度提高6.31%,重均分子质量提高20.21%,乙酰基含量提高77.07%,丙酮酸含量下降6.34%。结论改造后的菌株的黄原胶发酵产量和质量都较原始菌株有所提高。Purpose To improve the yield and quality of xanthan gum by overexpressing gumD in Xanthomonas campestris 58（Xc58）.MethodsBy PCR amplification,plasmid construction,triparental conjugation and other methods,pBBR-gumD was transformed into the original strain Xc58.ResultsCompared with Xc58,the recombinant strain Xc58-D has increased by 11.19% in the yield of xanthan gum,by 6.31% increased in viscosity,by 20.21 % increased in molecular weight,and by 77.07% increased in acetyl content,but 6.34% decreased in pyruvate content.ConclusionThe recombinant strain has a higher yield and improves the quality of xanthan gum.

关 键 词：黄原胶 gumD 重组菌株 

分 类 号：TQ460.38[化学工程—制药化工]