大豆异地衍生重组自交系群体遗传图谱的构建及比较  

Comparison of Two Genetic Maps of Soybean Constructed by RIL Populations Derived from Combinations of Peking×7605 under Two Ecological Sites

作　　者：洪雪娟[1] 侯金锋[1] 丁卉[1] 李永春[1] 盖钧镒[1] 邢邯[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《作物学报》2012年第4期614-623,共10页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2009CB118400);国家公益性行业(农业)科研专项(nycytx-004);江苏高校优势学科建设工程资助项目资助

摘　　要：以Peking×7605组合分别在山东济南和江苏南京衍生大豆重组自交系群体JN(RN)P7和NJ(RN)P7,利用145个在亲本之间表现多态的SSR引物和1个形态学标记BSC(黑色种皮性状)及JoinMap4.0软件构建了2张分别含27个和25个连锁群的大豆遗传图谱,其总长度分别为1574.80cM和1682.50cM,标记间平均距离分别是13.58cM和15.72cM,连锁群长度范围分别为17.30～127.40cM和20.10～137.50cM。所构建的2张图谱均与"公共图谱"对应性较好。2张图谱间整体上较为一致,但也存在诸多不同点。表明原本具有相同遗传背景的杂交后代,在不同生态环境选择压力下形成的重组自交系群体间遗传结构存在真实差异。Molecular linkage maps provide a powerful tool for the analysis of plant genome structure and function, and it is im- portant to determine the effect of ecological environment on soybean genetic map construction. Two soybean recombinant inbred line （RIL） populations named ＂JN（RN）P7＂ and ＂NJ（RN）P7＂ were tested in this study, which were derived from Peking x 7605 at Jinan and Nanjing, respectively. One hundred and forty-five SSR markers and one morphologic marker （BSC） which had polymorphism between parents were screened out to construct two genetic linkage maps using JoinMap 4.0 software. And we obtained two soybean genetic maps, which contained 27 and 25 of the linkage groups, respectively. The total length of two genetic maps was 1 574.80 and 1 682.50 cM, and the average distance between markers was 13.58 and 15.72 cM, respectively. The length of linkage group varied from 17.30 to 127.40 cM for JN（RN）P7, and from 20.10 to 137.50 cM for NJ（RN）P7. Both genetic maps were homologous with the public genetic map. The two maps constructed in this study were mostly coincident with each other, while certain differences were observed too, which indicated that there were some genetic structure differences between the two populations caused by the selection effect in various environments.

关 键 词：大豆 异地衍生重组自交系 遗传图谱 构建 真实差异 

分 类 号：S565.1[农业科学—作物学]