对溴磷及其对映体对人胎盘滋养层JEG-3细胞内分泌干扰的影响  

Enantioseletive effect of endocrine disruption by leptophos in JEG-3 cells

作　　者：陆颖冲[1] 张全[1] 王萃[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2012年第2期152-156,共5页Journal of Zhejiang University of Technology

基　　金：国家自然科学基金资助项目(21077094)

摘　　要：考察了有机磷农药对溴磷(Leptophos)的外消旋及其两个对映体对内分泌相关基因的干扰情况.探讨了以人类绒毛膜癌JEG-3细胞为模型,对溴磷的外消旋及其两个对映体暴露对产妇胎儿健康的潜在风险.研究通过采用实时定量PCR的方法,发现有机磷农药对溴磷的外消旋及其两个对映体对促性腺释放激素及其受体(GnRHI,GnRHII和GnRHR),胆固醇来源的雌激素合成以及胎儿在怀孕期间母体免疫(HLA-G)的相关基因表达都有不同程度的影响.并且(-)-Leptophos相对于(+)-Leptophos具有更强的内分泌干扰效应.In this study,we employed organophosphorus pesticides leptophos as a model compound,JEG-3 cell lines as in vitro model and assessed enantioselective disruption of leptophos towards the metabolic pathway of GnRH-mediated and estrogen,which derived from cholesterol synthesis.Besides,we desire to generally evaluate the potential risks of maternal-fetal health exposure to these compounds.The results revealed that organophosphorus pesticides leptophos exhibited significant enantioselectivity among the two enantiomers of leptophos in most of the genes,including GnRH and its receptor,genes corresponding to enzymes of the steroidogenesis pathway and HLA-G.Moreover,（-）-Leptophos showed a stronger endocrine disruption than its（＋）form in JEG-3 cell.

关 键 词：对溴磷 人类绒毛膜癌细胞 内分泌干扰 实时定量PCR 

分 类 号：R994.6[医药卫生—毒理学]