气相色谱法测定风油精中5种成分的含量  

Simultaneous determination of five compositions in Fengyoujing by GC

作　　者：陈海滨[1] 池文杰[1] 黄晓姗[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《海峡药学》2012年第3期56-58,共3页Strait Pharmaceutical Journal

摘　　要：目的研究提高风油精中薄荷脑、桉油精、樟脑、丁香酚、水杨酸甲酯5种成分的含量测定方法。方法色谱柱:Agilent INNOWAX石英毛细管柱(30m×0.32mm×0.25μm);载气为氮气;柱温采用程序升温:初始温度85℃,保持4min,以每分钟30℃的速率升至115℃,保持0min,以每分钟70℃的速率升至200℃,保持4min,以每分钟100℃的速率升至250℃,保持3min;检测器:FID;采用内标法测定。结果薄荷脑、桉油精、樟脑、水杨酸甲酯、丁香酚线性范围为0.34～17.22mg.mL-1(r=1.0000)、0.05～2.26mg.mL-1(r=1.0000),0.04～1.93mg.mL-1(r=0.9999),0.34～17.16mg.mL-1(r=1.0000)、0.04～1.80mg.mL-1(r=1.0000),平均回收率为96.17%、96.69%、101.14%、96.86%、98.02%。结论该方法经济、简单、准确且重现性好,可作为风油精的含量测定质量控制标准。OBJECTIVE To establish a GC method for simultaneous determination of Menthol,Cineol,Camphor,Methyl salicylate and Eugenol in Fengyoujing.METHODS Agilent INNOWAX column and FID detector were used.The column temperature was programmed as maintaining 85℃ for 4 minutes and rising to 115℃ at a rate of 30℃·min-1and maintaining for 0 minutes,then rising to 200℃ at a rate of 70℃·min-1and maintaining for 4 minutes,rising to 250℃ at a rate of 100℃·min-1and maintaining for 3 minutes.RESULTS The linear ranges of Menthol,Cineol,Camphor,Methyl salicylate and Eugenol were within 0.34~17.22mg·mL-1（r=1.0000）,0.05~2.26mg·mL-1（r=1.0000）,0.04~1.93mg·mL-1（r=0.9999）,0.34~17.16mg·mL-1（r=1.0000）,0.04~1.80mg·mL-1（r=1.0000）.The average recoveries of were 96.17%（RSD0.65%）,96.69%（RSD1.23%）,101.14%（RSD1.65%）,96.86%（RSD0.68%）,98.02%（RSD0.88%）,respectively.Conlusion：The method is economy,efficient and accurate with a good reproduction,it can be used to determine the five compositions in Fengyoujing.

关 键 词：风油精 气相色谱法 薄荷脑 桉油精 樟脑 丁香酚 水杨酸甲酯 

分 类 号：R927.2[医药卫生—药学]