一步提取-HPLC检测化妆品中氟喹诺酮类抗生素研究  

Determination and Extracted Directly of Fluoroqronolone in Cosmetics by HPLC

作　　者：徐万帮[1] 张莉[1] 刘亚雄[1] 蒋忠军[1] 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《广州化工》2012年第7期128-129,135,共3页GuangZhou Chemical Industry

基　　金：广东省药检系统课题(ZA20101526)

摘　　要：采用pH=6.0的NaAc-HAc缓冲液直接提取化妆品中氟喹诺酮类(氧氟沙星、诺氟沙星和环丙沙星)抗生素,建立反相高效液相色谱同时测定化妆品中氧氟沙星、诺氟沙星和环丙沙星的方法。以十八烷基硅烷键合硅胶为填充剂,以0.05 mol/L柠檬酸与1.0 mol/L醋酸铵(77∶1,用三乙胺调节pH至4.0)混合液——乙腈(85∶15)为流动相,检测波长为280 nm的条件下进行测定。结果表明氧氟沙星、诺氟沙星和环丙沙星的响应线性良好,回收率高。A HPLC method was established for the assay of fluoroqronolone antibiotics（ofloxacin,norfloxacin and ciprofloxacin）,which were extracted directly with the buffer of NaAc-HAc（pH 6.0）in cosmetics.The sample was analyzed on C18 with the buffer-acetonitrile（85：15）,which was consisted of 0.05 mol/L citric acid-acetic ammonium（77：1） and adjusting to pH 4.0 with triethanolamine,as the mobile phase and 280 nm as the detection wavelength.This method was proved to be highly effective and practicable.

关 键 词：直接提取 氟喹诺酮 化妆品 

分 类 号：R917[医药卫生—药物分析学]