降解苯并[a]芘的Bacillus pumilus strain Bap 9菌株的分离、鉴定与降解特性  

Isolation,Identification,and Biodegradation Characteristics of a Benzo[a]pyrene-degrading Bacterium Bacillus pumilus strain Bap9

作　　者：朱婷婷[1,2] 倪晋仁[1,2] 

机构地区：[1]北京大学深圳研究生院环境与能源学院,城市人居环境科学与技术重点实验室,深圳518055 [2]北京大学水沙科学教育部重点实验室,北京100871

出　　处：《应用基础与工程科学学报》2012年第2期169-178,共10页Journal of Basic Science and Engineering

基　　金："十一五"国家科技支撑计划项目(2006BAB04A14)

摘　　要：从长期受烧烤影响的土壤中,分离筛选出一株能利用苯并[a]芘作为唯一碳源和能源的高效降解菌Bap9,通过16S rRNA基因序列分析和部分生理生化特征分析,鉴定为Bacillus pumilus.摇瓶试验结果表明,菌株Bap9能在20d内将40mg/L的苯并[a]芘降解27.3%.通过对OD600和苯并[a]芘残留浓度的检测分析,考察了pH值、接种量和装液量对菌株生长和降解性能的影响.结果表明,菌株在pH值为6.0—9.0的条件下均能生长并能降解苯并[a]芘,降解的最适pH值为8.0,最佳接种量为5%,最佳装液量为10ml/50ml.A bacterial strain Bap9,which is able to utilize benzo[a]pyrene as sole carbon and energy source,was isolated from the polluted soil collected from the open-air barbecue market.The strain was identified as Bacillus pumilus according to its 16S rRNA sequence and physiological characteristic analysis.The experimental results showed that the strain could degrade 27.3% of benzo[a]pyrene（40 mg/L）after a 20d incubation.The influences of pH,inoculum quantity and aeration on the cell growth and BaP degradation were examined,through the analysis of OD600 and the BaP concentrations.Strain Bap9 could grow and utilize benzo[a]pyrene at pH 6.0—9.0,and its optimal condition for the benzo[a]pyrene degradation was pH 8.0,inoculum quantity 5%,aeration 10ml/50ml.

关 键 词：苯并[A]芘 微生物降解 BACILLUS pumilus 

分 类 号：X703[环境科学与工程—环境工程]